Description
Interleukin-13 (IL-13) is a cytokine produced by activated T lymphocytes which regulates the function of mouse macrophages and shares some biological functions with IL-4. Administration of mouse IL-13 to cultured mouse macrophages induces morphological changes (flattening, and development of processes), leads to increases in class II MHC antigen expression, and inhibits the synthesis of several proinflammatory cytokines (including IL-1β, IL-6, TNF, and IL-12). In contrast to human IL-13, mouse IL-13 does not appear to have activity for mouse B cells. Recombinant mouse IL-13 (mIL-13) has multiple glycosylated forms ranging in molecular weights from 11.3 to 15 kD, as detected by SDS-PAGE analysis.
Formulation and Purity:
Recombinant mouse IL-13 is supplied as a frozen aqueous buffered solution containing 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-13 is≥95% pure, as determined by SDS-PAGE, and an absorbance assay based on the Beers- Lambert law. The endotoxin level is ≤0.1 ng per µg of mouse IL-13, as measured in a chromogenic LAL assay.
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Avoid multiple freeze-thaws of product.Rapidly thaw and quick-spin product prior to use.Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Biological Activity
Activity Assay: Measured by proliferation usingTF-1 indicator cells
Specific Activity Range:0.6 - 5 x 10^7 Units*/mg
ED50: 0.2 - 1.5 ng/ml ; Observed dose response relationship: 50 fold
*Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
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