Anti-FLA-ST

January 20^th, 2020.This antibody has been removed from our catalog. However, you may contact us if you wish to order minimal quantities.

Detection and neutralization antibody to flagellin from S. typhimurium

Anti-FLA-ST is a mouse monoclonal antibody specific against flagellin from Salmonella typhimurium.

The antibody has been screened for the ability to neutralize FLA-ST-induced TLR5 activity. Anti-FLA-ST can be used for detection by ELISA and neutralization of purified or recombinant S. typhimurium flagellin.

The utility of this antibody for other applications has not been determined.

Specifications

Clonality: Monoclonal antibody

Clone: 18E11

Isotype: Mouse IgG1, kappa

Formulation: 0.2 µm filtered solution in 68 mM phosphate buffer with 91 mM glycine, 5% w/v saccharose and stabilizing agents

Application: Neutralization of FLA-ST-induced TLR5 activity, detection by ELISA

Specificity: flagellin from Salmonella typhimurium

Contents

• 100 µg purified anti-FLA-ST provided azide-free and lyophilized

Product is shipped at room temperature

Store lyophilized antibody at -20°C.

Product is stable for 6 months.

Details

Neutralization activity of hTLR5-Fc: Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene. After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.