Cell culture contamination control: Detection
Detection and identification of Mycoplasma
Venor® PCR Quantification Standards
Venor® Quantification Standards for Mycoplasma Detection from Minerva
Minerva Biolabs
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 52-0071 | Pseudomonas aeruginosa | Pseudomonas aeruginosa | £239.00 | Quantity | Add to Order |
| 52-0083 | Escherichia coli | each | £239.00 | Quantity | Add to Order |
| 52-0101 | Legionella pneumophila | £239.00 | Quantity | Add to Order | |
| 52-0112 | Mycoplasma orale | £239.00 | Quantity | Add to Order | |
| 52-0115 | Mycoplasma gallisepticum | Mycoplasma gallisepticum | £239.00 | Quantity | Add to Order |
| 52-0116 | Acholeplasma laidlawii | £239.00 | Quantity | Add to Order | |
| 52-0117 | Mycoplasma fermentans | £239.00 | Quantity | Add to Order | |
| 52-0119 | Mycoplasma pneumoniae | £239.00 | Quantity | Add to Order | |
| 52-0124 | Mycoplasma synoviae | Mycoplasma synoviae | £239.00 | Quantity | Add to Order |
| 52-0129 | Mycoplasma arginini | Mycoplasma arginini | £239.00 | Quantity | Add to Order |
| 52-0130 | Mycoplasma hyorhinis | £239.00 | Quantity | Add to Order | |
| 52-0164 | Spiroplasma citri | Spiroplasma citri | £239.00 | Quantity | Add to Order |
| 52-5571 | Bordetella pertussis | Bordetella pertussis | £239.00 | Quantity | Add to Order |
Related products
Venor® PCR Quantification Standards
Venor® Quantification Standards for Mycoplasma Detection from Minerva
Minerva Biolabs
The quantification standard is genomic DNA extracted from low passage and defined mycoplasma or legionella cultivated in liquid medium according to the European Pharmacopoeia or in Hayflick"s medium. The extraction of the DNA is done by column absorption methods and the DNA purified by phenol/chloroform extraction and ethanol precipitation. The concentration was determined photometrically and by real-time PCR. The DNA concentration was adjusted by dilution with DNA stabilizing buffer. Genomic DNA is used as a control template when DNA amplification tests by conventional PCR are performed (e.g. as contamination control in cell culturing). With the real-time PCR technology, this titrated DNA standard with a defined concentration of genome copies is used as calibrator to generate standard curves. Therefore the provided solution is diluted in a 10x dilution series with DNA-free water and used as a PCR sample. With the generated fluorescent data, the software provided with the real-time PCR instrument calculates a standard curve, which can be used to determine the DNA concentration of unknown samples.
Contents
1 vial (green cap) of Standard DNA, 100 µl, contains approx. 106 genomes/µl.3 vials (white cap) with 1 ml of DNA free water.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor® PCR Quantification Standards
Venor® Quantification Standards for Mycoplasma Detection from Minerva
Minerva Biolabs
Protocols
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor® PCR Quantification Standards
Venor® Quantification Standards for Mycoplasma Detection from Minerva
Minerva Biolabs
Dumke R. et al., (2017). Multi-center evaluation of one commercial and twelve in-house real-time PCR assays for detection of Mycoplasma pneumoniae. Diagnostic Microbiology & Infectious Disease, doi: 10.1016/j.diagmicrobio.2017.03.004.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor® PCR Quantification Standards
Venor® Quantification Standards for Mycoplasma Detection from Minerva
Minerva Biolabs
Applications
These DNA-Standards allow for the performance of a control reaction for PCR using qPCR instrumentation and to create a standard curve for quantification experiments, i.e. with Venor®Mp Mycoplasma pneumoniae Diagnostic Kit for qPCR. The DNA standards can be used as a general control to confirm PCR system performance using a qPCR instrument or for the validation of detection limits in respect to conventional PCR tests.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AthensResearch&Technology公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。
公司主要产品：一、凝血因子相关系列产品：包括多款从人血浆、血小板中分离纯化的凝血因子相关蛋白。二、酶及抑制剂系列产品：包括多款从人血浆、红细胞分离纯化的a-抗糜蛋白酶、a1/2抗胰蛋白酶、抗凝血酶、C1酯酶抑制因子、Calpain-1等酶及其抑制剂产品。三、免疫球蛋白系列产品：包括多款从人血浆、骨髓瘤、初乳以及狗和小鼠血浆中分离纯化的免疫球蛋白产品。四、脂蛋白及载脂蛋白系列产品：包括包括多款从人血浆中分离纯化的载脂蛋白及脂蛋白（高密度、中等密度、低密度、极低密度脂蛋白）产品。五、低内毒素蛋白系列产品：包括多款适宜于细胞培养的低内毒素脂蛋白、免疫球蛋白及转铁蛋白产品。六、乳蛋白系列产品：包括从人乳中分离纯化的乳白蛋白、乳铁传递蛋白及分泌型免疫球蛋白A。七、中性粒细胞源蛋白系列产品：包括多款纯化分离自人中性粒细胞的Azurocidin 、CathepsinG及Lactoferrin等蛋白产品。八、血小板相关系列产品：包括纯化分离自人血小板的血小板因子4及凝血酶致敏蛋白。九、转铁蛋白及脱铁转铁蛋白（APOTRANSFERRINS）系列产品：包括多款纯化分离自人、大小鼠、狗及食蟹猴血浆中的转铁蛋白/脱铁转铁蛋白，可应用于细胞或组织培养。十、抗体系列产品：包括多款兔、大鼠、羊源的多克隆抗体产品。
ebiomall.com
