Enzymes for Molecular Biology: Enzymes for Molecular Biology
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EN510250 | Exonuclease VII, E. coli | 250U | £194.00 | Quantity | Add to Order |
| EX4425K | Exonuclease III, E. coli | 200U/µl 25,000U | £176.00 | Quantity | Add to Order |
Related products
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
Exonuclease III digests duplex DNA in a 3"Æ5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results.
Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction factors including temperature, ionic strength, template sequence, and enzyme-to-DNA ratios,3,4 each template must be optimised using sample digestions to achieve the desired excision rate.
Exonuclease III is not active on 3"-protruding ends of 4 bases or more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3"-DNA phosphatase, and apurinic DNA endonuclease activities.
Unit Definition
One unit catalyses the release of 1nmole of acid-soluble nucleotides from double-stranded calf thymus DNA in 30 minutes at 37°C in 33mM Tris-acetate, pH 7.8, 66mM potassium acetate, 10mM magnesium acetate, and 0.5mM DTT.
Storage Buffer
50% glycerol containing 50 mM Tris-HCl, pH 7.5, 0.1M NaCl, 0.1mM EDTA, 1mM DTT, and 0.1% Triton® X-100.
Exonuclease III 10X Reaction Buffer
330mM Tris-acetate, pH 7.8, 660mM potassium acetate, 100mM magnesium acetate, and 5mM DTT.
Quality Control
Exonuclease III is free of detectable exogenous RNase, endonuclease, and single-stranded exonuclease activities.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
Protocols for: Exonuclease III, E. coli and Exonuclease VII
Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens’s product page, where the latest protocol is available.
Please note this will open a new page or window on your computer.
Exonuclease III Protocol
(catalogue number EX4405K / EX4425K)
Please note: all protocols off site are the responsibility of the products supplier
Exonuclease VII Protocol
(catalogue numberEN510100 / EN510250)
Please note: all protocols off site are the responsibility of the products supplier
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
References
Exonuclease III
- Sambrook, J. et al. (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York.
- Vandeyar, M.A. (1988) Gene 65, 129.
- Luckow, B. et al. (1987) Nucleic Acids Res. 15, 417.
- Richardson, C.C. et al. (1964) J. Biol. Chem. 239, 251.
- Weiss, B. (1976) J. Biol. Chem. 251, 1896.
- Rogers, S.G. and Weiss, B. (1980) Meth. Enzymol. 65, 201.
- Guo, L.H. and Wu, R. (1982) Nucleic Acids Res. 10, 2065.
Exonuclease VII
- Li, H. et al. (1991) Nucleic Acids Res. 19, 3139.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Exonuclease III, E. coli and Exonuclease VII
Exonuclease III digests duplex DNA in a 3" → 5" direction from a blunt end, 5"-overhang or nick, producing stretches of single-stranded DNA on the opposite strand.
BioSearch Tech (Lucigen/Epicentre)
Applications
- Production of intermediates for site-directed mutagenesis.1-3
- Production of strand-specific radiolabeled probes.4
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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