Enzymes for Molecular Biology: Enzymes for Molecular Biology
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.
BioSearch Tech (Lucigen/Epicentre)
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| RJ411250 | Rec J Exonuclease | 250U 10 U/µl | £231.00 | Quantity | Add to Order |
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.
BioSearch Tech (Lucigen/Epicentre)
Rec J Exonuclease, derived from E. coli, catalyzes removal of deoxyribonucleoside monophosphates from single-stranded DNA in a 5"- 3" direction. Its activity is dependent on Mg 2. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.
Unit Definition
One unit is the amount of enzyme that catalyzes release of 1 nmol of acid-soluble nucleotides from activated single-stranded calf thymus DNA in 30 minutes at 37°C.
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 0.1 M NaCl, and 0.1%Triton® X-100.
10X Reaction Buffer
330 mM Tris-acetate (pH 7.8), 660 mM potassium acetate, 100 mM magnesium, acetate, and 5.0 mM DTT.
Quality Control
Rec J Exonuclease is free of detectable RNase and contaminating DNA exo- and endonucleaseactivities.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.
BioSearch Tech (Lucigen/Epicentre)
Protocols for: Rec J Exonuclease, E. coli
Rec J Exonuclease Protocol
(catalogue number RJ411050 / RJ411250)
Please note: all protocols off site are the responsibility of the products supplier
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.
BioSearch Tech (Lucigen/Epicentre)
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Rec J Exonuclease
Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´?3´ direction and is used for the removal of primers from completed PCR and degradation of single-stranded linear DNA in dsDNA and plasmid preps.
BioSearch Tech (Lucigen/Epicentre)
Applications
- Removal of primers from completed PCR.
- Degradation of single-stranded linear DNA in dsDNA and plasmid preps
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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