Enzymes for Molecular Biology: Enzymes for Molecular Biology
T4 RNA Ligase 2, Deletion Mutant
T4 RNA Ligase 2, Deletion Mutant, also known as T4Rnl2(1-249)
BioSearch Tech (Lucigen/Epicentre)
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| LR2D11310K | T4 RNA Ligase 2, Deletion Mutant | 10kU | £217.00 | Quantity | Add to Order |
| LR2D1132K | T4 RNA Ligase 2, Deletion Mutant | 2kU | £63.00 | Quantity | Add to Order |
Related products
T4 RNA Ligase 2, Deletion Mutant
T4 RNA Ligase 2, Deletion Mutant, also known as T4Rnl2(1-249)
BioSearch Tech (Lucigen/Epicentre)
Ligate single-stranded adenylated DNA or RNA oligos to RNA targets for a variety of applications.
- Ligate single-stranded adenylated DNA or RNA oligos to RNA targets
- Use this enzyme to prepare cDNA libraries for small-RNA transcriptome analysis such as RNA-Seq
- Perform linker ligation for miRNA cloning applications
T4 RNA Ligase 2, Deletion Mutant, also known as T4Rnl2(1-249), is used to ligate single-stranded adenylated DNA or RNA oligonucleotides to small RNAs for cloning or next-generation RNA sequencing. The preadenylated 5´ ends of DNA or RNA are ligated to the 3´ ends of RNA. Unlike the full-length enzyme, T4Rnl2(1-249) is unable to adenylate the 5´ end of the substrate in the presence of ATP. However, it can use a preactivated donor (App-DNA or App-RNA) and join it to the 3´ end of an acceptor; thus, performing the ligation reaction in the absence of ATP which prevents circularization and other undesirable bimolecular reactions.
The enzyme is available in 2,000 and 10,000-Unit sizes at a concentration of 200 U/µl. The enzyme is supplied with a 10X Reaction Buffer.
Unit Definition: One unit is the amount of enzyme required to give 50% ligation of a 22-mer RNA to the preadenylated end of a 17-mer DNA when both oligos are annealed to a complementary 39-mer DNA strand in 30 minutes at 37°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM dithiothreitol (DTT), and 0.1% Triton® X-100.
T4 RNA Ligase 2, Deletion Mutant, 10X Reaction Buffer: 500 mM Tris-HCl (pH 7.5), 20 mM MgCl2, and 10 mM DTT.
Activity Assay: The unit definition assay is performed in a reaction containing 50 mM Tris-HCl (pH 7.5), 2 mM MgCl2, 1 mM DTT and 0.4 µg of equimolar mix of 22-mer, 17-mer and 39-mer oligonucleotides, and varying amounts of enzyme.
Quality Control: T4 RNA Ligase 2, Deletion Mutant, is free of detectable DNA exo- and endonuclease, and RNase activities.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 RNA Ligase 2, Deletion Mutant
T4 RNA Ligase 2, Deletion Mutant, also known as T4Rnl2(1-249)
BioSearch Tech (Lucigen/Epicentre)
PROTOCOL
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
T4 RNA Ligase 2, Deletion Mutant
T4 RNA Ligase 2, Deletion Mutant, also known as T4Rnl2(1-249)
BioSearch Tech (Lucigen/Epicentre)
Applications
- Preparing cDNA libraries for small-RNA transcriptome analysis such as RNA-Seq.
- Optimal linker ligation for miRNA cloning.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AthensResearch&Technology公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。
公司主要产品：一、凝血因子相关系列产品：包括多款从人血浆、血小板中分离纯化的凝血因子相关蛋白。二、酶及抑制剂系列产品：包括多款从人血浆、红细胞分离纯化的a-抗糜蛋白酶、a1/2抗胰蛋白酶、抗凝血酶、C1酯酶抑制因子、Calpain-1等酶及其抑制剂产品。三、免疫球蛋白系列产品：包括多款从人血浆、骨髓瘤、初乳以及狗和小鼠血浆中分离纯化的免疫球蛋白产品。四、脂蛋白及载脂蛋白系列产品：包括包括多款从人血浆中分离纯化的载脂蛋白及脂蛋白（高密度、中等密度、低密度、极低密度脂蛋白）产品。五、低内毒素蛋白系列产品：包括多款适宜于细胞培养的低内毒素脂蛋白、免疫球蛋白及转铁蛋白产品。六、乳蛋白系列产品：包括从人乳中分离纯化的乳白蛋白、乳铁传递蛋白及分泌型免疫球蛋白A。七、中性粒细胞源蛋白系列产品：包括多款纯化分离自人中性粒细胞的Azurocidin 、CathepsinG及Lactoferrin等蛋白产品。八、血小板相关系列产品：包括纯化分离自人血小板的血小板因子4及凝血酶致敏蛋白。九、转铁蛋白及脱铁转铁蛋白（APOTRANSFERRINS）系列产品：包括多款纯化分离自人、大小鼠、狗及食蟹猴血浆中的转铁蛋白/脱铁转铁蛋白，可应用于细胞或组织培养。十、抗体系列产品：包括多款兔、大鼠、羊源的多克隆抗体产品。
ebiomall.com
