Lab Reagents: Smart Buffers and Reagents
Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago"s SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility
Tris-EDTA (TE) Buffer 10x, pH 7.4
TE buffer is composed by Tris, a buffering agent and EDTA, a chelating agent. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
Medicago AB
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| MC-12-9154-10 | Tris-EDTA buffer (TE) 10x pH 7.4, 1000 ml | 10 pouches | £113.00 | Quantity | Add to Order |
Tris-EDTA (TE) Buffer 10x, pH 7.4
TE buffer is composed by Tris, a buffering agent and EDTA, a chelating agent. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
Medicago AB
Product description
TE buffer is composed by Tris, a buffering agent and EDTA, a chelating agent. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
Medicago’s TE buffer is supplied as exactly pre-weighed powder in pouches, giving 1000 ml of 0.1 M Tris-HCl, 0.010 M Tris-EDTA buffer with pH 7.4 at 25°C when dissolved in nuclease free water or deionized water.
Features
- pH adjusted for DNA/RNA work
- Sterilize by autoclaving or filtration
- Contains chelating agent
- 10x pre-weighed powder mix in pouches
- Dissolve and go for greater convenience
Directions for use
Empty one pouch of the TE buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the buffer solution is ready to use.
Shipping and storage
TE buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date.
Specifications
Chemical: Analytical grade
Format: Exactly pre-weighed powder mix
Concentration: 0.1 M Tris-HCl, 0.010 M EDTA
Volume: 1000 ml
pH: 7.4 ± 0.05 at 25°C
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Tris-EDTA (TE) Buffer 10x, pH 7.4
TE buffer is composed by Tris, a buffering agent and EDTA, a chelating agent. EDTA has the ability to prevent degradation of DNA and RNA by chelating magnesium- or other divalent metal ions.Tris-EDTA-based solutions break protein cross-links and can therefore unmask antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. Treatment with TE buffer enhances the staining intensity of antibodies in the immuno-histochemical detection of certain proteins.
Medicago AB
Applications
- Storing and diluting DNA and RNA
- Breaking protein-links in immuno-histochemistry procedures
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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