Oligo Synthesis : CEPs
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2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3800-02 | 2"-F-A-ANA CE Phosphoramidite | 0.25g | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3800-90 | 2"-F-A-ANA CE Phosphoramidite | 100umole | £140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3820-02 | 2"-F-G-ANA CE Phosphoramidite | 0.25g | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3820-90 | 2"-F-G-ANA CE Phosphoramidite | 100umole | £140.00£133.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3830-02 | 2"-F-U-ANA CE Phosphoramidite | 0.25g | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3830-05 | 2"-F-U-ANA CE Phosphoramidite | 0.5g | £400.00£380.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
Catalog Number: 10-3800-xx
Description: 2"-FANA-A-CE Phosphoramidite
| 5"-Dimethoxytrityl-N6-benzoyl-2"-deoxy-2"-fluoroarabinoadenosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C47H51FN7O7P | M.W.: 875.93 | F.W.: 331.2 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time recommended. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 1-2 days |
2’-F-Arabinonucleic Acid (2’-F-ANA)
Arabinonucleosides are epimers of ribonucleosides with the chiral switch being at the 2’ position of the sugar residue. 2’-F-ANA adopts a more DNA-like B-type helix conformation, not through the typical C2’-endo conformation but, rather, through an unusual O4’-endo (east) pucker. However, the presence of the electronegative fluorine leads to a still significant increase (DTm1.2° C/mod) in melting temperature per modification.1 2’-F-ANA-containing oligonucleotides exhibit very high binding specificity to their targets. Indeed, a single mismatch in a 2’-F-ANA – RNA duplex leads to a DTm of -7.2 °C and in a 2’-F-ANA - DNA duplex a DTm of -3.9 °C.2
The presence of fluorine at the 2’ position in 2’-F-ANA leads to increased stability to hydrolysis under basic conditions relative to RNA and even 2’-F-RNA.1,3 The stability of 2’-F-ANA to nucleases also makes this a useful modification for enhancing the stability of oligonucleotides in biological environments.2 2’-F-ANA hybridizes strongly to target RNA and, unlike most 2’ modifications, induces cleavage of the target by RNase H. Phosphorothioate (PS) 2’-F-ANA is routinely used in these applications due to its increased nuclease resistance. Alternating 2’-F-ANA and DNA units provide among the highest potency RNase H-activating oligomers. Both the “altimer” and “gapmer” strand architectures consistently outperform PS-DNA and DNA/RNA gapmers.4
siRNA oligos were found to tolerate the presence of 2’-F-ANA linkages very well. High potency gene silencing was demonstrated5 with siRNA chimeras containing 2’-F-RNA and/or LNA and 2’-F-ANA. The high efficacy of these chimeras was attributed to the combination of the rigid RNA-like properties of 2’-F-RNA and LNA with the DNA-like properties of 2’-F-ANA.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
MSDS
Glen Report 22.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
- E Viazovkina, M M Mangos, M I Elzagheid, and M H Damha, Curr Protoc Nucleic Acid Chem., 2002, chapter 4, Unit 4 15
- J K Watts, and M J Damha, Can J Chem., 2008, 86, 641-656
- J K Watts, A Katolik, J Viladoms, and M J Damha, Org Biomol Chem., 2009, 7, 1904-10
- A Kalota, et al., Nucleic Acids Res., 2006, 34, 451
- G F Deleavey, et al., Nucleic Acids Res., 2010, 38, 4547-4557
- J K Watts, et al., Nucleic Acids Res., 2007, 35, 1441-1451
- T Dowler, et al., Nucleic Acids Res., 2006, 34, 1669-1675
2"-F-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows:
WO/2009/146556 (siRNA)
WO 03064441 and WO 0220773 (antisense)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
2"-F-ANA is covered by intellectual property. Key patents covering siRNA and antisense applications are as follows:
WO/2009/146556 (siRNA)
WO 03064441 and WO 0220773 (antisense)
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-F-ANA CE Phosphoramidites
2"-F-Arabinonucleic Acid CE Phosphoramidites for A, Bz-C, G and U
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3800-90 | 100µmoles | .088grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-3800-02 | 0.25grams | .25grams | 2.85 | 81.67 | 49 | 30.63 | 22.27 | 16.33 | 4.08 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3800-90 | 100µmoles | .088grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-3800-02 | 0.25grams | .25grams | 4.26 | .07 | 78.8 | 49.25 | 35.82 | 4.93 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3800-90 | 100µmoles | .088grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-3800-02 | 0.25grams | .25grams | 4.26 | .07 | 80.4 | 50.25 | 36.55 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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