Oligo Synthesis : CEPs
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5"-Fluorescein Phosphoramidite
5"-Fluorescein Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-5901-02 | 5"-Fluorescein Phosphoramidite | 0.25g | £460.00£437.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5901-90 | 5"-Fluorescein Phosphoramidite | 100µmoles | £172.00£163.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5901-95 | 5"-Fluorescein Phosphoramidite | 50µmoles | £88.00£83.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-Fluorescein Phosphoramidite
5"-Fluorescein Phosphoramidite
Glen Research
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5"-Fluorescein Phosphoramidite
Catalog Number: 10-5901-xx
Description: 5"-Fluorescein Phosphoramidite
| 6-(3",6"-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C46H58N3O10P | M.W.: 843.95 | F.W.: 537.46 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days, <90% efficient after 4 days |
| Please Note: This product or portions thereof is manufactured under license from GE Healthcare Bio-Sciences Corp. under US Patent Numbers 5,371,241, 5,721,355, and/or 5,583,236 |
FLUORESCEIN LABELLING
5’-Fluorescein phosphoramidite contains no 4,4’-dimethoxytrityl (DMT) group and can be added only once at the 5’-terminus, thereby terminating synthesis. This product is prepared using the 6-carboxyfluorescein derivative. The tetrachloro-, hexachloro-and dichloro-dimethoxy-fluorescein phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers. Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling. The analogous structure, 6-Fluorescein Phosphoramidite, prepared using 6-FAM, is also available, along with 6-Fluorescein Serinol Phosphoramidite. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.
We offer five fluorescein supports. Fluorescein CPG has traditionally been used to add the fluorescein label at the 3’-terminus. The analogous structure, 3’-(6-Fluorescein) CPG, prepared using 6-FAM, is now also available, along with 6-Fluorescein Serinol CPG. We also offer 3’-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM). Both are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. 3’-(6-FAM) CPG allows effective blockage of the 3’-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generates the fluorescein labelled oligos.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Fluorescein Phosphoramidite
5"-Fluorescein Phosphoramidite
Glen Research
MSDS
Glen Report 8.2
Glen Report 12.1
Glen Report 21.2
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Fluorescein Phosphoramidite
5"-Fluorescein Phosphoramidite
Glen Research
5"-Fluorescein Phosphoramidite
| 6-(3",6"-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C46H58N3O10P | M.W.: 843.95 | F.W.: 537.46 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days, <90% efficient after 4 days |
| Please Note: This product or portions thereof is manufactured under license from GE Healthcare Bio-Sciences Corp. under US Patent Numbers 5,371,241, 5,721,355, and/or 5,583,236 |
| Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20oC. Do not store crude fluorescently labeled oligonucleotides in ammonia. |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 8.2: 5" LABELLING - FLUORESCEIN AND CYANINE DYES, BIOTIN |
| Glen Report 12.1: POLY-PAK PURIFICATION OF LABELLED PROBES |
| Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides |
Frequently Asked Technical Question
QUESTION: What are the relative extinction coefficients of 5"-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html
REFERENCE(S):Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
QUESTION: Does AMA or methylamine cause any degradation to fluorescein or fluorescein-type dyes such as FAM or FITC?
RESPONSE:Response: While AMA (Ammonium hydroxide/40% Methylamine 1:1 v/v) is considered compatible with fluorescein, the use of methylamine when deprotecting a Fluorescein-labeled oligo does lead to a small amount of degradation, which is characterized by a the appearance of a late-eluting peak by RP HPLC that shows no visible fluorescein absorbance. With standard deprotection conditions (AMA 10 minutes at 65 C) the amount of degradation is approximately 5%. The impurity is not detected with AMA at RT for 2 hours.
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 2.96 | 85.33 | 51.2 | 32 | 23.27 | 17.07 | 4.27 |
| 10-5901-90 | 100µmoles | .084grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5901-95 | 50µmoles | .042grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 4.42 | .067 | 82 | 51.25 | 37.27 | 5.13 | |
| 10-5901-90 | 100µmoles | .084grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5901-95 | 50µmoles | .042grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 4.42 | .067 | 83.6 | 52.25 | 38 | ||
| 10-5901-90 | 100µmoles | .084grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-5901-95 | 50µmoles | .042grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-Fluorescein Phosphoramidite
5"-Fluorescein Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 2.96 | 85.33 | 51.2 | 32 | 23.27 | 17.07 | 4.27 |
| 10-5901-90 | 100µmoles | .084grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5901-95 | 50µmoles | .042grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 4.42 | .07 | 82 | 51.25 | 37.27 | 5.13 | |
| 10-5901-90 | 100µmoles | .084grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5901-95 | 50µmoles | .042grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5901-02 | 0.25grams | .25grams | 4.42 | .07 | 83.6 | 52.25 | 38 | ||
| 10-5901-90 | 100µmoles | .084grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5901-95 | 50µmoles | .042grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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