Oligo Synthesis : CEPs
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5-Me-2"-deoxyZebularine-CE Phosphoramidite
5-Me-2"-deoxyZebularine-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1061-02 | 5-Me-2"-deoxyZebularine-CE Phosphoramidite | 0.25g | £800.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1061-90 | 5-Me-2"-deoxyZebularine-CE Phosphoramidite | 100µmoles | £320.00£304.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1061-95 | 5-Me-2"-deoxyZebularine-CE Phosphoramidite | 50µmoles | £168.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5-Me-2"-deoxyZebularine-CE Phosphoramidite
5-Me-2"-deoxyZebularine-CE Phosphoramidite
Glen Research
| 5-Me-2"-deoxyZebularine-CE Phosphoramidite |
Catalog Number: 10-1061-xx
Description: 5-Me-2"-deoxyZebularine-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-methyl-pyrimidin-2-one-2"-deoxyriboside, 3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H49N4O7P | M.W.: 728.82 | F.W.: 288.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Ammonium Hydroxide for 24 hrs at room temperature or as required by nucleobases. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
THERAPEUTIC Nucleosides
Cytosine Arabanoside (Ara-C) is an anti-viral drug which has achieved limiteduse. Its effect on DNA structure and activity can be investigated byincorporating it into synthetic oligonucleotides.
Zebularine (pyrimidin-2-one ribonucleoside) is a cytidine analogue that actsas a DNA demethylase inhibitor, as well as a cytidine deaminase inhibitor. Thisstructure is very active biologically and Zebularine is now used as a potentanti-cancer drug. A 2’-deoxynucleoside analogue of Zebularine,5-methyl-pyrimidin-2-one, 2’-deoxynucleoside, has been used to probe theinitiation of the cellular DNA repair process by making use of its mildlyfluorescent properties. This combination of biological activity and fluorescenceproperties would make 5-Me-2"-deoxyZebularine a strong addition to our array ofnucleoside analogues.
Cytosine-5-methyltransferases are found in everything from archaebacteria tomammals and when the regulation of cytosine-5-methyltransferases goes awry,cancer can result. The mechanism of action for this family of enzymes involvesattack of a cysteine thiol group on the C6 position of cytosine, leading to atransient dihydrocytosine intermediate, which then facilitates the nucleophilicattack by C5 on the activated methyl group of the S-adenosyl-L-methioninecofactor. As with many enzymes, the intermediate can be trapped using a suicidesubstrate and 5-fluoro-cytosine has been used extensively in this role. Analternate strategy is to use a transition-state mimic that binds to the activesite with high affinity. An excellent candidate was found in5-aza-5,6-dihydrocytosine. Despite not being covalently bound to the enzyme, itwas found1,2 to be a more potent inhibitor ofcytosine-5-methyltransferases than 5-fluoro-cytosine. 5-Aza-5,6-dihydro-dC iscompatible with standard oligonucleotide synthesis and deprotection conditionsand is an excellent tool for use in methyltransferase research.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-2"-deoxyZebularine-CE Phosphoramidite
5-Me-2"-deoxyZebularine-CE Phosphoramidite
Glen Research
MSDS
Glen Report 16.2: STILL MORE NEW PRODUCTS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-2"-deoxyZebularine-CE Phosphoramidite
5-Me-2"-deoxyZebularine-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1061 | 5-Me-dZ | 314 | 4.8 | 218 | 8.6 | 1.8 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1061-02 | 0.25grams | .25grams | 3.43 | 101 | 60.6 | 37.88 | 27.55 | 20.2 | 5.05 |
| 10-1061-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1061-95 | 50µmoles | .036grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1061-02 | 0.25grams | .25grams | 5.12 | .07 | 96 | 60 | 43.64 | 6 | |
| 10-1061-90 | 100µmoles | .073grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1061-95 | 50µmoles | .036grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1061-02 | 0.25grams | .25grams | 5.12 | .07 | 97.6 | 61 | 44.36 | ||
| 10-1061-90 | 100µmoles | .073grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1061-95 | 50µmoles | .036grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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