Oligo Synthesis : CEPs
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5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1018-02 | 5-Me-dC Brancher Phosphoramidite | 0.25g | £404.00£383.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1018-90 | 5-Me-dC Brancher Phosphoramidite | 100µmoles | £164.00£155.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
| 5-Me-dC Brancher Phosphoramidite |
Catalog Number: 10-1018-xx
Description: 5-Me-dC Brancher Phosphoramidite
| 5"-Dimethoxytrityl-N4-(O-levulinyl-6-oxyhexyl)-5-Methyl-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C51H68N5O10P | M.W.: 942.10 | F.W.: 402.36 |
Diluent: Anhydrous Acetonitrile |
| Coupling: dC Brancher reacts in a manner identical to normal phosphoramidites. |
| Levulinyl Deprotection:The levulinyl protecting group can be selectively removed without cleavage of the oligonucleotide from the CPG by treatment with 0.5 M Hydrazine hydrate in 1:1 pyridine/acetic acid.[Note hydrazine hydrate is a violent poison that is both volatile and readily absorbed through skin]Fit the column with syringes and push the solution back and forth across the column.Let sit for 15 minutes at room temperature.Rinse the column with 1.5 mL of 1:1 pyridine/acetic acid (3x) and then 1.5 mL of ACN (3x).Dry CPG under a stream of argon and proceed with the synthesis of the branching sequence.If a non-branching control is desired, simply deprotect in ammonium hydroxide as required by the nucleobases.Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
BRANCHING PHOSPHORAMIDITE
A branching monomer is required to construct comb-likeoligonucleotide probes. The developers of the comb system from ChironCorporation evaluated3 several protecting groups for the branch pointand chose levulinyl (LEV), which is specifically removed using a reagentcontaining hydrazine hydrate, acetic acid and pyridine.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
MSDS
| Glen Report 18.1: Synthesis of Branched DNA with a Comb Structure |
| Glen Report 24.1: Technical Brief - Compatibility of UltraMild Monomers or Ac-dC with Hydrazine Reagent |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5-Me-dC Brancher Phosphoramidite
5-Me-dC Brancher Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 2.65 | 75 | 45 | 28.13 | 20.45 | 15 | 3.75 |
| 10-1018-90 | 100µmoles | .094grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 3.96 | .07 | 72.8 | 45.5 | 33.09 | 4.55 | |
| 10-1018-90 | 100µmoles | .094grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1018-02 | 0.25grams | .25grams | 3.96 | .07 | 74.4 | 46.5 | 33.82 | ||
| 10-1018-90 | 100µmoles | .094grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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