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Athens Research/Cambio - Excellence in Molecular Biology/50µmoles/13-1013-95

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13-1013-95

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Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

ACT Trimer Phosphoramidite

Glen Research

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
13-1013-90	ACT Trimer Phosphoramidite	100µmoles	£680.00£646.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order
13-1013-95	ACT Trimer Phosphoramidite	50µmoles	£340.00£323.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView Offer	Quantity	Add to Order

ACT Trimer Phosphoramidite

Glen Research

ACT Trimer Phosphoramidite

Structure

Catalog Number: 13-1013-xx

Description: ACT Trimer Phosphoramidite

5"-Dimethoxytrityl-N6-benzoyl-2"-deoxyAdenosine-3"->5"-o-chlorophenyl-phosphoryl-N4-benzoyl-2"-deoxyCytidine-3"->5"-o-chlorophenyl-phosphoryl-2"-deoxyThymidine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C₈₅H₈₇Cl₂N₁₂O₂₁P₃	M.W.: 1774.5

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)

Coupling:15 minute coupling time recommended.

Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 2-3 days

Trimer Phosphoramidites

In principle, the simplest approach for oligonucleotide-directedmutagenesis would be the use of trimer phosphoramidites. Of the 64 possiblecombinations of codons, only 20 codons would be required to cover the 20 aminoacids, although, in practice, several codons will likely be duplicated dependingon the organism. Our trimers use the protection scheme described^1-3 by Kayushin et al. There is a concern that the sequence of the trimers has to beverified. For example, CAT coding for histidine, has to be differentiated fromTAC, coding for tyrosine. These two trimers have virtually identicallipophilicity and their identity cannot be clearly confirmed by HPLC. Thisproblem has been solved⁴ using HPLC electrospray mass spectrometricanalysis of the trimers, which provides data confirming molecular weight andsequence.

In Table 1, the trimers, their coding amino acid and theirreaction factor (RF) are listed. The reaction factor is critical since thetrimers will likely be mixed and they have differing reactivity in the couplingreaction. RF for AAC is 1.0 and for TAC is 1.6. Therefore, 1.6 equivalents ofTAC are needed for every 1.0 equivalent of AAC for equal coupling. Mixtures caneasily be made using equimolar solutions or the molecular weight of each trimerhas to be used to generate the appropriate weights of each trimer to use ifmixing by weight. An example of the preparation of a mixture of all 20 trimersis shown in the right column of Table 1 and completed in the footnotes.

All of the trimers are now available individually so thatresearchers can prepare custom mixtures. A mixture of all 20 trimers designed toproduce equal coupling of all 20 is also available. If you require customproduction of a specific mixture, please e-mail support@glenresearch.com for aquotation and projected delivery.

TABLE 1: TRIMER CODING AND PHYSICAL PARAMETERS

Trimer	Amino Acid	abbreviation	MW	RF	MWxRF	mg/10µmol(adjusted for RF)
AAA	Lys	K	1911.5	1.1	2102.65	21.0 (11)
AAC	Asn	N	1887.5	1.0	1887.50	18.9 (10)
ACT	Thr	T	1774.5	1.3	2306.85	23.1 (13)
ATC	Ile	I	1774.5	1.2	2129.40	21.3 (12)
ATG	Met	M	1780.5	1.3	2314.65	23.1 (13)
CAG	Gln	Q	1869.5	2.0	3739.00	37.4 (20)
CAT	His	H	1774.5	1.9	3371.55	33.7 (19)
CCG	Pro	P	1845.5	1.8	3321.90	33.2 (18)
CGT	Arg	R	1756.5	1.1	1932.15	19.3 (11)
CTG	Leu	L	1756.5	1.2	2107.80	21.1 (12)
GAA	Glu	E	1893.5	1.9	3597.65	36.0 (19)
GAC	Asp	D	1869.5	1.3	2430.35	24.3 (13)
GCT	Ala	A	1756.5	1.5	2634.75	26.3 (15)
GGT	Gly	G	1762.5	1.1	1938.75	19.4 (11)
GTT	Val	V	1667.5	1.9	3168.25	31.7 (19)
TAC	Tyr	Y	1774.5	1.6	2839.20	28.4 (16)
TCT	Ser	S	1661.4	1.3	2159.82	21.6 (13)
TGC	Cys	C	1756.5	1.5	2634.75	26.3 (15)
TGG	Trp	W	1762.5	2.4	4230.00	42.3 (24)
TTC	Phe	F	1661.4	1.3	2159.82	21.6 (13)
=530.0 mg

Example of Preparation of Trimer Mixture

Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer fromthe right column. Dissolve the trimer mix in dichloromethane (highest gradepossible; acid-free). Evaporate to dryness to produce a homogenous mixture ofall 20 trimers.

Example of Preparation of Trimer Mixture for the Synthesizer

Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) ofthe trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v toproduce a 0.10N solution of trimers, ready for use in a synthesizer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ACT Trimer Phosphoramidite

Glen Research

MSDS

Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION

Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ACT Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	LV40	LV200	40nm	0.2µm	1µm	10µm
Cat.No.	PackSize	Grams/Pack	0.1M Dil.(mL)	Approximate Number of Additions
13-1013-90	100µmoles	.177grams	1	20	12	7.5	5.45	4	1
13-1013-95	50µmoles	.089grams	.5	3.33	2	1.25	.91	.67	.17
Expedite
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	50nm	0.2µm	1µm	15µm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
13-1013-90	100µmoles	.177grams	1.5	.07	23.6	14.75	10.73	1.48
13-1013-95	50µmoles	.089grams	.75	.07	8.6	5.38	3.91	.54
Beckman
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Molarity	30nm	200nm	1000nm
Cat.No.	PackSize	Grams/Pack	Dilution(mL)	Approximate Number of Additions
13-1013-90	100µmoles	.177grams	1.5	.07	25.2	15.75	11.45
13-1013-95	50µmoles	.089grams	.75	.07	10.2	6.38	4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AthensResearch&Technology公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。

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