Oligo Synthesis : CEPs
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BHQ-2-dT
BHQ-2-dT
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-5942-02 | BHQ-2-dT | 0.25g | £980.00£703.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5942-90 | BHQ-2-dT | 100µmoles | £568.00£399.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5942-95 | BHQ-2-dT | 50µmoles | £292.00£201.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
BHQ-2-dT
BHQ-2-dT
Glen Research
Catalog Number: 10-5942-xx
Description: BHQ-2-dT
| 5"-Dimethoxytrityloxy-5-[(N-4""-carboxyethyl-4""-(N-ethyl)-4"-(4-Nitro-phenyldiazo)-2"-methoxy-4"-methoxy-azobenzene)-aminohexyl-3-acrylimido]-2"-deoxyUridine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C73H87N12O15P | M.W.: 1403.53 | F.W.: 962.91 |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling: 15 minute coupling time recommended |
| Deprotection: Complete the deprotection using the protocol required by the nucleobases.Note, the use of methylamine in ultrafast deprotection protocols will result in degradation to the dye and is not recommended. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: "Black Hole Quencher", "BHQ-0", "BHQ-1", "BHQ-2" and "BHQ-3" are trademarks of Biosearch Technologies, Inc., Novato, CA.The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc..Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user.They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged. |
Black Hole Quencher Dyes
With the growing popularity of red and near-infrared dyes, we areoffering the Black Hole QuencherTM dyes (BHQs), whose physical properties aredetailed in Table 1. BHQ dyes are robust dark quenchers that very nicelycomplement our existing product line. They are compatible with ammoniumhydroxide deprotection, exhibit excellent coupling efficiencies, have largeextinction coefficients and are completely non-fluorescent. Their absorbancesare well-tuned to quench a variety of popular fluorophores – even those far intothe red, such as Cy3 and Cy5. The dark quencher most typically used in aMolecular Beacon is Dabcyl. Because the quenching does not involve FRET, thereis little, if any, dependence upon donor-acceptor spectral overlap. In acomprehensive paper by Marras, Kramer and Tyagi,1 the ability ofBHQ-1 and BHQ-2 to quench 22 different fluorophores was evaluated. For shorterwavelength fluorophores such as fluorescein, the quenching efficiency wasroughly the same as Dabcyl (91% – 93%). However, for dyes emitting in the farred, such as Cy5, the BHQ dyes were far superior – quenching the Cy5 with 96%efficiency, compared to 84% with Dabcyl. This may reflect the BHQ’s ability toform stable, non-fluorescent complexes which can be a plus even in FRET probes.Indeed, recent work suggests that these non-fluorescent complexes will form evenin the absence of a hairpin stem structure used by MolecularBeacons.2
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BHQ-2-dT
BHQ-2-dT
Glen Research
Material Safety Data Sheet
Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides
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BHQ-2-dT
BHQ-2-dT
Glen Research
Intellectual Property
"Black Hole Quencher", "BHQ-0", "BHQ-1", "BHQ-2" and "BHQ-3" are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc.. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.
References
(1) S.A.E. Marras, F.R. Kramer, and S. Tyagi, Nucleic Acids Res., 2002, 30, E122.
(2) M.K. Johansson, H. Fidder, D. Dick, and R.M. Cook, J Am Chem Soc, 2002, 124, 6950-6956.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
BHQ-2-dT
BHQ-2-dT
Glen Research
Frequently Asked Technical Question
QUESTION: What are the physical properties of the various black hole quenchers?
RESPONSE:
| Quencher | lmax (nm) | E260 (L/mol.cm) | Emax (L/mol.cm) |
| BHQ-0 | 493 | 7,700 | 34,000 |
| BHQ-1 | 534 | 8,000 | 34,000 |
| BHQ-2 | 579 | 8,000 | 38,000 |
| BHQ-3 | 672 | 13,000 | 42,700 |
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BHQ-2-dT
BHQ-2-dT
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 1.78 | 46 | 27.6 | 17.25 | 12.55 | 9.2 | 2.3 |
| 10-5942-90 | 100µmoles | .14grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5942-95 | 50µmoles | .07grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 2.66 | .07 | 46.8 | 29.25 | 21.27 | 2.93 | |
| 10-5942-90 | 100µmoles | .14grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5942-95 | 50µmoles | .07grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5942-02 | 0.25grams | .25grams | 2.66 | .07 | 48.4 | 30.25 | 22 | ||
| 10-5942-90 | 100µmoles | .14grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5942-95 | 50µmoles | .07grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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