Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
Chemical Phosphorylation Reagent
Chemical Phosphorylation Reagent
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1900-02 | Chemical Phosphorylation Reagent | 0.25g | £128.00£121.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1900-90 | Chemical Phosphorylation Reagent | 100µmoles | £40.00£38.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
Chemical Phosphorylation Reagent
Chemical Phosphorylation Reagent
Glen Research
| Chemical Phosphorylation Reagent |
Catalog Number: 10-1900-xx
Description: Chemical Phosphorylation Reagent
| 2-[2-(4,4"-Dimethoxytrityloxy)ethylsulfonyl]ethyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C34H45N2O7PS | M.W.: 656.77 | F.W.: 79.98 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved.(Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions.In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Note: The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
CHEMICAL PHOSPHORYLATION
Chemical Phosphorylation Reagent is most commonly used tophosphorylate the 5’-terminus of an oligonucleotide. Although this product isalso successful in 3’-phosphorylation, 3’-Phosphate CPG allows directpreparation of oligonucleotides with a 3’-phosphate group. ChemicalPhosphorylation Reagent II contains a DMT group on a side chain which is stableto base cleavage and can be left on the oligonucleotide for use in RPpurification. The DMT group is later removed with aqueous acid and the sidechain is eliminated after brief treatment with aqueous ammonium hydroxide toyield the 5’-phosphate.1 Solid CPR II is similar in performance to CPR II but itis easier to prepare aliquots since it is a powder. Many researchers treatsynthesis supports with a hindered base (e.g., diethylamine,diisopropylethylamine, or DBU) post-synthesis to eliminate and remove thecyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ isremoved from the support and is not available to alkylate dT residues at the N3position in the oligos. Since the sulfonylethyl group in 3’-Phosphate CPG isalso susceptible to ß-elimination leading to oligo cleavage, this technique isnot compatible with 3’-phosphate CPG. Using CPR II CPG, which is base labile butdoes not support ß-elimination, the cyanoethyl groups can be removed from theoligo prior to cleavage and base deprotection. ABI-style vials and columns aresupplied unless otherwise requested.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Chemical Phosphorylation Reagent
Chemical Phosphorylation Reagent
Glen Research
Material Safety Data Sheet
| Glen Report 8.2: 5"-PHOSPHORYLATION - NOW COMPATIBLE WITH DMT-ON PURIFICATION |
| Glen Report 12.1: PRODUCT UPDATE - WHICH CHEMICAL PHOSPHORYLATION REAGENT? |
| Glen Report 20.1: New Products |
| Glen Report 23.1: Technical Brief - Chemical Phosphorylation Ð considering the options |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Chemical Phosphorylation Reagent
Chemical Phosphorylation Reagent
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 3.81 | 113.67 | 68.2 | 42.63 | 31 | 22.73 | 5.68 |
| 10-1900-90 | 100µmoles | .066grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .07 | 107.2 | 67 | 48.73 | 6.7 | |
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1900-02 | 0.25grams | .25grams | 5.68 | .07 | 108.8 | 68 | 49.45 | ||
| 10-1900-90 | 100µmoles | .066grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
AthensResearch&Technology公司位于美国乔治亚州雅典市，从1986年以来一直致力于纯化高纯度，高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白，血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来，世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。我们提供优质，可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务，AthensResearch&Technology公司能够根据您的项目要求提供无与伦比的试剂。
公司主要产品：一、凝血因子相关系列产品：包括多款从人血浆、血小板中分离纯化的凝血因子相关蛋白。二、酶及抑制剂系列产品：包括多款从人血浆、红细胞分离纯化的a-抗糜蛋白酶、a1/2抗胰蛋白酶、抗凝血酶、C1酯酶抑制因子、Calpain-1等酶及其抑制剂产品。三、免疫球蛋白系列产品：包括多款从人血浆、骨髓瘤、初乳以及狗和小鼠血浆中分离纯化的免疫球蛋白产品。四、脂蛋白及载脂蛋白系列产品：包括包括多款从人血浆中分离纯化的载脂蛋白及脂蛋白（高密度、中等密度、低密度、极低密度脂蛋白）产品。五、低内毒素蛋白系列产品：包括多款适宜于细胞培养的低内毒素脂蛋白、免疫球蛋白及转铁蛋白产品。六、乳蛋白系列产品：包括从人乳中分离纯化的乳白蛋白、乳铁传递蛋白及分泌型免疫球蛋白A。七、中性粒细胞源蛋白系列产品：包括多款纯化分离自人中性粒细胞的Azurocidin 、CathepsinG及Lactoferrin等蛋白产品。八、血小板相关系列产品：包括纯化分离自人血小板的血小板因子4及凝血酶致敏蛋白。九、转铁蛋白及脱铁转铁蛋白（APOTRANSFERRINS）系列产品：包括多款纯化分离自人、大小鼠、狗及食蟹猴血浆中的转铁蛋白/脱铁转铁蛋白，可应用于细胞或组织培养。十、抗体系列产品：包括多款兔、大鼠、羊源的多克隆抗体产品。
ebiomall.com
