Oligo Synthesis : CEPs
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Coumarin Azide
Coumarin Azide
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 50-2004-90 | Coumarin Azide | 100umole | £280.00£266.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 50-2004-92 | Coumarin Azide | 25umole | £92.00£87.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
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Coumarin Azide
Coumarin Azide
Glen Research
Coumarin Azide
Catalog Number: 50-2004-xx
Description: Coumarin Azide
| 3-Azido-7-Hydroxycoumarin | ||
| Formula: C9H5N3O3 | M.W.: 203.15 | |
Diluent: Not Applicable |
| Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: |
Conjugation using Click Chemistry(CONT.)
Glen Research is collaborating with baseclick Gmbh to offer a variety ofinteresting alkyne phosphoramidites. We offer some azide products and are nowembarking on an expansion of our azide catalog. Our strategy is to offer firstour most popular labels for general interest and, subsequently, we will addazide products that are not compatible with phosphoramidite chemistry.
Biotin is still our most commonly used label and biotinTEG, with itshydrophilic triethylene glycol spacer, is the most popular biotin product.Desthiobiotin is a biotin analogue that is well captured by streptavidin but thecaptured product can be easily released by applying a biotin solution to thestreptavidin beads. 6-FAM is our most popular fluorescein derivative and weoffer azides of both 6-FAM and pivaloyl-protected 6-FAM for situations wheresubsequent reactions require the 6-FAM to be protected. In both 6-FAM products,the hydrophilic TEG spacer is again used. The azides are offered in 25 and 100µmole packs for convenient oligonucleotide labelling.
7-Hydroxycoumarin, also known as umbelliferone, is a highly fluorescent,pH-sensitive fluorophore that emits in the blue region of the spectrum. However,its fluorescence is strongly quenched if the hydroxyl is alkylated orphosphorylated, making it useful in high-throughput screening for phosphatasesand lipases. Interestingly, it was found that the 3-azido derivative is alsohighly quenched but, upon reaction with an alkyne in the presence of copper toform the triazole, the fluorescence is restored.1 The clicked coumarin emits ata lambda max of 480 nm and absorbs at 358 nm.
HEX and TET are two of our most popular fluorescein-based dyes for labellingoligonucleotides. We are happy to offer 6-HEX and 6-TET Azides for use in clickconjugations.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Coumarin Azide
Coumarin Azide
Glen Research
Material Safety Data Sheet
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Coumarin Azide
Coumarin Azide
Glen Research
1. The Glen Report, 2010, 22, 1-4
2. J Gierlich, G A Burley, P M Gramlich, D M Hammond and T Carell, Org Lett, 2006, 8, 3639-42
3. F Seela and V R Sirivolu, Chem Biodivers, 2006, 3, 509-14
4. P M E Gramlich, S Warncke, J Gierlich and T Carell, Angewandte Chemie-International Edition, 2008, 47, 3442-3444
5. P M E Gramlich, C T Wirges, A Manetto and T Carell, Angewandte Chemie-International Edition, 2008, 47, 8350-8358
6. K Li, L A Lee, X Lu and Q Wang, BioTechniques, 2010, 49, 525-7
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Coumarin Azide
Coumarin Azide
Glen Research
Literature Highlights
| Glen Report 23.1: New Products - Click Chemistry Update |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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