Oligo Synthesis : CEPs
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DBCO-dT-CE Phosphoramidite
DBCO-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1539-02 | DBCO-dT-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1539-90 | DBCO-dT-CE Phosphoramidite | 100 µmole | £388.00£368.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1539-95 | DBCO-dT-CE Phosphoramidite | 50 µmole | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
DBCO-dT-CE Phosphoramidite
DBCO-dT-CE Phosphoramidite
Glen Research
DBCO-dT-CE Phosphoramidite
Catalog Number: 10-1539-xx
Description: DBCO-dT-CE Phosphoramidite
| 5"-Dimethoxytrityl-5-[(6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-capramido-N-hex-6-yl)-3-acrylimido]-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C69H80N7O11P | M.W.: 1214.57 | F.W.: 773.77 |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling: 12 minute coupling time is recommended. |
| Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65 °C or 17 hours at room temperature. AMA (ammonium hydroxide/40% methylamine 1:1) for 2 hours at room temperature will give acceptable results. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
COPPER-FREE CLICK CHEMISTRY
At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
- Simple to use
- Stable in solution on the synthesizer
- Stable to ammonium hydroxide and AMA
- Excellent click performance in 17 hours or less at room temperature
From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5’-DBCO-TEG Phosphoramidite for preparing oligos with a 5’-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
DBCO-dT-CE Phosphoramidite
DBCO-dT-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 24.2: New Product - DBCO-dT for Copper-Free Click Chemistry
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
DBCO-dT-CE Phosphoramidite
DBCO-dT-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1539-95 | 50µmoles | .061grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-1539-90 | 100µmoles | .121grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1539-02 | 0.25grams | .25grams | 2.06 | 55.33 | 33.2 | 20.75 | 15.09 | 11.07 | 2.77 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1539-95 | 50µmoles | .061grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-1539-90 | 100µmoles | .121grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1539-02 | 0.25grams | .25grams | 3.07 | .07 | 55 | 34.38 | 25 | 3.44 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1539-95 | 50µmoles | .061grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-1539-90 | 100µmoles | .121grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1539-02 | 0.25grams | .25grams | 3.07 | .07 | 56.6 | 35.38 | 25.73 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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