+,BioPORTER® Protein Delivery Reagent BioPORTER® Protein Delivery Reagent BioPORTER Protein Transfection Reagent (Delivery)

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genlantis/BioPORTER® Protein Delivery Reagent/24 rxn kit, BP502401 $334 (+239)</option><option value=24 single/use tubes kit, BP502424

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genlantis/BioPORTER® Protein Delivery Reagent/24 rxn kit, BP502401 $334 (+239)

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Efficient protein delivery into a wide variety of cell types
2X more effective than electroporation
Efficient delivery of a variety of proteins, peptides, and other macromolecules
Fast and Easy Protocol

BioPORTER^® Protein Delivery Reagent is a unique lipid formulation that allows direct translocation of proteins into living cells. BioPORTER Protein Delivery kits provide researchers with a quick and easy method to study protein function without the need for cloning and DNA transfection. After capturing the protein, the reagent transports the lipid protein complex into the target cell. The delivered proteins retain their structure and function while leaving the transduced cells unharmed. The reagent is especially useful when studying protein function in cells that are difficult to transfect using traditional DNA protein transfection reagents.

ContentsBioPORTER kits are available as either a bulk reagent or as pre-dispensed and dried in convenient single-use QuikEase vials.

Dry BioPORTER Protein Delivery Reagent (1 vial for Cat. No. BP502401, 4 vials for Cat. No. BP509604, 24 single use vials for Cat. No. BP502424, 96 single-use vials for Cat. No. BP509696)

β-galactosidase Control Protein (1 vial x 10 μg)

FITC-labeled Goat IgG Control Protein (1 vial x 10 μg)

StorageBioPORTER Protein Delivery Reagent kits are shipped frozen. For maximum stability, store all biological reagents and components at -20ºC upon receipt. The reagent is stable for 1 year when stored at the recommended temperature.

BioPORTER Protein Delivery Kit

24 rxn kit, BP502401
24 single-use tubes kit, BP502424
96 rxn kit, BP509604
96 single-use tubes kit, BP509696
FITC Antibody Control 10 ul, ABFITC01
FITC Antibody Control 6 x 10 ul, ABFITC06
B-gal Control Protein 10 ul, BGALCP01
B-gal Control Protein 6 x 10 ul, BGALCP06

Patents pending

New Citations Correction of Impaired Calmodulin Binding to Ryr2 as a Novel Therapy of Lethal Arrhythmia in the Pressure-Overloaded Heart Failure Nakamura, Y.,et al (2016) HRS 14(1): 120-127. Link

Deletion of Na+/H+ exchanger regulatory factor 2 represses colon cancer progress by suppression of Stat3 and CD24 Yoshida, M.,et al (2016) Am. J. Physiol. Gastrointest. Liver Physiol. 310(8): G586–G59. Link

The Dynamics and Turnover of Tau Aggregates in Cultured Cells: INSIGHTS INTO THERAPIES FOR TAUOPATHIES Guo, J.L.,et al (2016) J. Biol. Chem. 291(25): 13175–13193. Link

High Affinity CaM, but Not Normal Affinity CaM Can Prevent Arrhythmias in CPVT Which Mutation is Located at CaM-like Domain of RyR2Nishimura, S.,et al (2015) AHA 132(3): 2967-2978 Link

Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola VirusAdu-Gyamfi, E.,et al (2015) J. Virol. 89(18): 9440-945. Link

Cytotoxicity of Human Endogenous RetrovirusK–Specific T Cells toward Autologous Ovarian Cancer CellsRycaj, K.,et al (2014) Clin. Cancer Res. 21(2): 471–8 Link

Extracellular Monomeric Tau Protein Is Sufficient to Initiate the Spread of Tau Protein PathologyMichel, C.H.,et al (2014) J. Biol. Chem.289(2): 956 –96. Link

AAA Peroxins and Their Recruiter Pex26p Modulate the Interactions of Peroxins Involved in Peroxisomal Protein ImportTamura, S.,et al (2014) J. Biol. Chem. 289(35): 24336 –24346. Link

Dissociation of Calmodulin From RyR2 Causes Diastolic Ca2+ leak and Induces Lethal Arrhythmias in Pressure-overload Heart FailureKato, T.,et al (2014) AHA 130(2): 1524-1539. Link

Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cellsHsu, C.W.,et al (2014) J. Biomed. Mater. Res. 102(11):4037-4043 Link

BioPORTER Protein Delivery Reagent Cell Line Citations: A549, B16-F0, BHK-21, BN16, C2C12, C6, CA9-22, Calu-3, CHO-K1, Cos-1, COS-7, HCN2A, HEK 293, HeLa, HeLa-S3, HepG2, Heu127, HL60, HLE-B3, Huh-7, HUVEC, Jurkat, K562, Ki-Ras 267, LNCaP, MEG-01,NIH-3T3, OK, Osteosarcoma Cell Line, PC-12, Primary Cortical Neurons, Primary Pancreatic Islets of Langerhans Epithelial beta Cells, Primary Neutrophils, Primary LEC, Primary Polymorphonuclear Leukocytes, Primary Neutrophils, Primary Adventitial Fibroblasts, Primary Cardiac Myocytes, Primary Vascular Smooth Muscle Cells, Primary Ventricular Muscle, Primary Cortical Neurons, Primary Astroglia Capillary Endothelial Cells, Primary AML, Primary Bronchial Epithelial Cells, Primary CML, Primary Cortical Neurons, Primary Eye Lens Epithelium, Primary Microvascular Endothelial Cells, Primary Neutrophils, Primary Retina, Primary Retinal Growth Cone, rBCEC4, SH-SY5Y, UM-SCC-23

BioPORTER^® Protein Delivery Kit provides researchers with a quick and easy method to study protein function without the need for cloning and DNA transfection. BioPORTER^® reagent lipid captures proteins and transports them inside the target cells. The delivered proteins retain their structure and function while leaving the transduced cells unharmed. BioPORTER^® kit is especially useful when studying protein function in cells that are difficult to transfect using traditional DNA transfection reagents.

For additional speed and convenience, The BioPORTER Reagent product line is also available in pre-coated single-use BioPORTER® QuikEase™ tubes. QuikEase tubes save hours of time by eliminating the need to coat the BioPORTER Reagent onto individual vials.

BP502401-509604 BioPORTER® Manual (pdf)BP502424-BP509696 BioPORTER® QuikEase Manual (pdf)BP502401-509604 BioPORTER® Brochure (pdf)BP502401-509604 BioPORTER® Citations (pdf)BP502401-509604 BioPORTER® Data (pdf)BP502401-509604 BioPORTER® FAQs (pdf)

Cell Line	Cell Type	Source	Genlantis Product	Citation
A549	Lung Carcinoma	Human	BioPORTER Protein Delivery Reagent	Anestal, K. and Arnéér, E.S.J. (2003) Rapid induction of cell death by selenium compromised thioredoxin reductase 1 but not by the fully active enzyme containing selenocysteine. J. Biol. Chem: 278: 15966 - 15972.
A549	Lung Carcinoma	Human	BioPORTER Protein Delivery Reagent	Haince, J-F., Kozlov, S., Dawson, V.L., Dawson, T.M., Hendzel, M.J., Lavin, M.F. and Poirier, G.G. (2007) Ataxia Telangiectasia Mutated (ATM) Signaling Network Is Modulated by a Novel Poly(ADP-ribose)-dependent Pathway in the Early Response to DNA-damaging Agents. J. Biol. Chem. 282: 16441-16453.
B16-F0	Melanoma	Mouse	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
BHK-21	Kidney Fibroblast	Hamster	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
BN16	Yolk Sac Carcinoma	Rat	BioPORTER Protein Delivery Reagent	Nagai, J., Christensen, E. I., Morris, S. M., Willnow, T. E., Cooper, J. A. and Nielsen, R. (2005) Mutually dependent localization of megalin and Dab2 in the renal proximal tubule. AJP: Renal; 289(3): p. F569-F576. (Delivery of IgG into BN16)
C2C12	Myoblast	Mouse	BioPORTER Protein Delivery Reagent	Casciola-Rosen, L., Garcia-Calvo, M., Bull, H.G., Becker, J.W., Hines, T., Thornberry, N.A. and Rosen, A. (2007) Mouse and human granzyme B have distinct tetrapeptide specificities and abilities to recruit the Bid pathway. J Biol Chem 282: 4545 - 4552. (Granzyme B delivery)
C2C12	Myoblast	Mouse	BioPORTER Protein Delivery Reagent	Fernando, P., Kelly, J.F., Balazsi, K., Slack, R.S., and Megeney, L.A. (2002) Caspase 3 activity is required for skeletal muscle differentiation Proc. Natl. Acad. Sci. 99: 11025 - 11030.
C6	Glioma	Rat	BioPORTER Protein Delivery Reagent	Zassler, B., Blasig, IE and Humpel, C. (2005) Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells. J Neurooncol. 71(2): p. 127.
CA9-22	Gingival Squamous Cell Carcinoma	Human	BioPORTER Protein Delivery Reagent	Yamamoto, K., Tominaga, K., Sukedai, M., Okinaga, T., Iwanaga, K., Nishihara, T. and Fukuda, J. (2004) Delivery of cytolethal distending toxin B induces cell cycle arrest and apoptosis in gingival squamous cell carcinoma in vitro. Eur J Oral Sci 112(5): p. 445.
Calu-3	Lung Adenocarcinoma	Human	BioPORTER Protein Delivery Reagent	Liedtke, C.M., Hubbard, M. and Wang, X. (2003) Stability of actin cytoskeleton and PKC-? binding to actin regulate NKCC1 function in airway epithelial cells. Am J Physiol Cell Physiol 284: 487 - 496.
Calu-3	Lung Adenocarcinoma	Human	BioPORTER Protein Delivery Reagent	Liedtke, C.M., Raghuram, V., Yun, C.C. and Wang, X. (2004) Role of a PDZ1 domain of NHERF1 in the binding of airway epithelial RACK1 to NHERF1. AJP: Cell 286(5): p. C1037-C1044.
Calu-3	Lung Adenocarcinoma	Human	BioPORTER Protein Delivery Reagent	Smallwood, N.D., Hausman, B.S., Wang, X. and Liedtke, C.M. (2005) Involvement of N-terminus of PKC{delta} in binding to F-actin during activation of Calu-3 airway epithelial NKCC1. AJP: Cell 288: 906 - 912.
CHO-K1	Ovary	Chinese Hamster	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
CHO-K1	Ovary	Chinese Hamster	BioPORTER Protein Delivery Reagent	Zheng, X., Lundberg, M., Karlsson, A. and Johansson, M. (2003) Lipid-mediated protein delivery of suicide nucleoside kinases. Cancer Res. 63: 6909-6913.
Cos-1	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Clegg, J.S. (2007) Protein Stability in Artemia Embryos During Prolonged Anoxia. Biol. Bull. 212(1): p. 74-81.
COS-1	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Collins, CH and Clegg, JS (2004)¿A small heat-shock protein, p26, from the crustacean Artemia protects mammalian cells (Cos-1) against oxidative damage. Cell Biol Int 1 28(6): p. 449.
COS-7	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Braun, A., Pinyol, R., Dahlhaus, R., Koch, D., Fonarev, P., Grant, B.D., Kessels, M.M. and Qualmann, B. (2005) EHD Proteins Associate with Syndapin I and II and Such Interactions Play a Crucial Role in Endosomal Recycling Mol. Biol. Cell. 16(8): p. 3642-3658. (Delivery of IgG)
COS-7	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Fenster, S.D., Kessels, M.M., Qualmann, B., Chung, W.J., Nash, J., Gundelfinger, E.D. and Garner, C.C. (2003) Interactions between Piccolo and the actin/dynamin-binding protein Abp1 link vesicle endocytosis to presynaptic active zones. J. Biol. Chem. 278: 20268.
COS-7	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Kessels, M.M. and Qualmann, B. (2002) Syndapins integrate N-WASP in receptor-mediated endocytosis. EMBO J. 21: 6083 - 6094.
Cos-7	Kidney	Green Monkey	BioPORTER Protein Delivery Reagent	Marques, C., Guo, W., Pereira, P., Taylor, A., Patterson, C., Evans, P.C. and Shang, F. (2006) The triage of damaged proteins: degradation by the ubiquitin-proteasome pathway or repair by molecular chaperones. FASEB J. 20(6):741-3.
HCN2A	Cortical Neurons	Human	BioPORTER Protein Delivery Reagent	Shimura, H., Miura-Shimura, Y. and Kosik, K.S. (2004) Binding of Tau to Heat Shock Protein 27 Leads to Decreased Concentration of Hyperphosphorylated Tau and Enhanced Cell Survival. J. Biol. Chem. 279(17): p. 17957-17962
HEK 293	Embryonic Kidney	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 35110.
HeLa	Cervical Carcinoma	Human	BioPORTER Protein Delivery Reagent	Fortugno, P., Wall, N.R., Giodini, A., O"Connor, D.S., Plescia, J., Padgett, K.M., Tognin, S., Marchisio, P.C. and Altieri, D.C. (2002) Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J. Cell Sci. 115: 575 - 585.
HeLa-S3	Cervical Carcinoma	Human	BioPORTER Protein Delivery Reagent	Fortugno, P., Beltrami, E., Plescia, J., Fontana, J., Pradhan, D., Marchisio, P.C., Sessa, W.C. and Altieri, D.C. (2003) Regulation of survivin function by Hsp90. PNAS 100: 13791 - 13796.
HeLa-S3	Cervical Carcinoma	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
HepG2	Hepatocellular Carcinoma	Human	BioPORTER Protein Delivery Reagent	Shao, H., Cheng, HY, Cook, R.G. and Tweardy, DJ (2003) Identification and Characterization of Signal Transducer and Activator of Transcription 3 Recruitment Sites within the Epidermal Growth Factor Receptor. Cancer Res 63: 3923.
Heu127	T-Cell Lymphoma	Human	BioPORTER Protein Delivery Reagent	Thiery, J., Dorothée, G., Haddada, H., Echchakir, H., Richon, C., Stancou, R., Vergnon, I., Benard, J., Mami-Chouaib, F. and Chouaib, S. (2003) Potentiation of a Tumor Cell Susceptibility to Autologous CTL Killing by Restoration of Wild-Type p53 Function. J. Immunol. 170: 5919 - 5926.
HL60	Acute Myeloid Leukemia	Human	BioPORTER Protein Delivery Reagent	McGrath, M.S., Rosenblum, M.G., Philips, M.R. and Scheinberg, D.A. (2003) Immunotoxin Resistance in Multidrug Resistant Cells. Cancer Res. 63: 72 - 79.
HLE-B3	Lens Epithelial Cells	Human	BioPORTER Protein Delivery Reagent	Fernando, M.R., Lechner, J.M., Lofgren, S., Gladyshev, V.N. and Lou, M.F. (2006) Mitochondrial thioltransferase (glutaredoxin 2) has GSH-dependent and thioredoxin reductase-dependent peroxidase activities in vitro and in lens epithelial cells FASEB J. 20(14): p. 2645-2647. (Delivery of Human Grx2, β-galactosidase, catalase)
Huh-7	Hepatoma	Human	BioPORTER Protein Delivery Reagent	Rabe, B., Glebe, D. and Kann, M. (2006) Lipid-mediated introduction of hepatitis B virus capsids into nonsusceptible cells allows highly efficient replication and facilitates the study of early infection events. J Virol 80(11): p. 5465.
HUVEC	Umbilical Vein Endothelial	Human	BioPORTER Protein Delivery Reagent	Kakoki, M., Kim, H-S, Edgell, C-J.S., Maeda, N., Smithies, O. and Mattson, D.L. (2006) Amino acids as modulators of endothelium-derived nitric oxide. Am. J. Physiol. Renal Physiol. 291(2): F297-F304.
Jurkat	T-Cell Lymphoma	Human	BioPORTER Protein Delivery Reagent	Azuma, Y., Kurusu, Y., Sato, H., Higai, K. and Matsumoto, K. (2007) Increased Expression of Lewis X and Y Antigens on the Cell Surface and FUT 4 mRNA during Granzyme B -Induced Jurkat Cell Apoptosis. Biol Pharm Bull 30(4): p. 655.
Jurkat	T-Cell Lymphoma	Human	BioPORTER Protein Delivery Reagent	Casciola-Rosen, L., Garcia-Calvo, M., Bull, H.G., Becker, J.W., Hines, T., Thornberry, N.A. and Rosen, A. (2007) Mouse and human granzyme B have distinct tetrapeptide specificities and abilities to recruit the Bid pathway. J Biol Chem 282: 4545 - 4552.
Jurkat	T-Cell Lymphoma	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
K562	Chronic Myeloid Leukemia	Human	BioPORTER Protein Delivery Reagent	Hostetter, D.R., Loeb, C.R.K., Chu, F. and Craik, C.S. (2007) Hip Is a Pro-survival Substrate of Granzyme B. J. Biol. Chem. 282(38): 27865-27874.
Ki-Ras 267?	Prostate Epithelium	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
LNCaP	Prostate Cancer	Human	BioPORTER Protein Delivery Reagent	Loeb, C.R.K., Harris, J.L. and Craik, C.S. (2006) Granzyme B Proteolyzes Receptors Important to Proliferation and Survival, Tipping the Balance toward Apoptosis. J. Biol. Chem. 281(38): 28326-28335.
MEG-01	Megakaryocyte	Human	BioPORTER Protein Delivery Reagent	Clarke, M.C.H., Savill, J., Jones, D.B., Noble, B.S. and Brown, S.B. (2003) Compartmentalized megakaryocyte death generates functional platelets committed to caspase-independent death J. Cell Biol. 160: 577 - 587.
NIH-3T3	Fibroblast	Mouse	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
NIH-3T3	Fibroblast	Mouse	BioPORTER Protein Delivery Reagent	Zhao, R., Fu, X., Teng, L., Li, Q. and Zhao, Z.J. (2003) Blocking the Function of Tyrosine Phosphatase SHP-2 by Targeting Its Src Homology 2 Domains. J. Biol Chem. 278: 42893-42898.
OK	Kidney	Opossum	BioPORTER Protein Delivery Reagent	Khundmiri, S.J., Dean, W.L., McLeish, K.R. and Lederer, E.D. (2005) Parathyroid Hormone-mediated Regulation of Na+-K+-ATPase Requires ERK-dependent Translocation of Protein Kinase C{alpha}. J. Biol. Chem. 280(10): p. 8705-8713.
Osteosarcoma Cell Line	Osteosarcoma	Not Identified	BioPORTER Protein Delivery Reagent	Zheng, X., Lundberg, M., Karlsson, A. and Johansson, M. (2003) Lipid-mediated protein delivery of suicide nucleoside kinases. Cancer Res. 63: 6909-6913.
PC-12	Pheochromocytoma	Rat	BioPORTER Protein Delivery Reagent	Anantharam, V., Kitazawa, M., Wagner, J., Kaul, S., and Kanthasamy, A.G., (2002) Caspase-3-Dependent Proteolytic Cleavage of Protein Kinase C Is Essential for Oxidative Stress-Mediated Dopaminergic Cell Death after Exposure to Methylcyclopentadienyl Manganese Tricarbonyl. J. Neurosci. 22:1738 - 1751.
PC-12	Pheochromocytoma	Rat	BioPORTER Protein Delivery Reagent	Gil, G.A., Bussolino, D.F., Portal, M.M., Pecchio, A.A., Renner, M.L., Borioli, G.A., Guido, M.E. and Caputto, B.L. (2004) c-Fos Activated Phospholipid Synthesis Is Required for Neurite Elongation in Differentiating PC12 Cells.Mol. Biol. Cell 15: 1881 - 1894.
PC-12	Pheochromocytoma	Rat	BioPORTER Protein Delivery Reagent	Kanno, T., Yamamoto, H., Yaguchi, T., Hi, R., Mukasa, T., Fujikawa, H., Nagata, T., Yamamoto, S., Tanaka, A. and Nishizaki, T. (2006) The newly synthesized linoleic acid derivative DCP-LA selectively activates PKC-epsilon possibly by binding to the phosphatidylserine binding site J. Lipid Res. 47: 1146-1156.
PC-12	Pheochromocytoma	Rat	BioPORTER Protein Delivery Reagent	Murakami, Y., Aizu-Yokota, E., Sonoda, Y., Ohta, S. and Kasahara, T. (2007) Suppression of Endoplasmic Reticulum Stress-induced Caspase Activation and Cell Death by the Overexpression of Bcl-xL or Bcl-2. J. Biochem. (Tokyo). 141(3): p. 401-410. (Antibody Delivery)
Primary	Cortical Neurons	Mouse	BioPORTER Protein Delivery Reagent	Andrabi, S.A., Kim, N.S., Yu, S-W, Wang, H., Koh, D.W., Sasaki, M., Klaus, J.A., Otsuka, T., Zhang, Z., Koehler, R.C., Hurn, P.D., Poirier, G.G., Dawson, V.L. and Dawson, T.M. (2006) Poly(ADP-ribose) (PAR) polymer is a death signal. Proc. Natl. Acad. Sci. 103: 18308 - 18313.
Primary	Pancreatic Islets of Langerhans Epithelial beta Cells	Syrian Golden Hamster	BioPORTER Protein Delivery Reagent	Cheng, H., Grodnitzky, J.A., Yibchok-anun, S., Ding, J. and Hsu, W.H. (2005) Somatostatin Increases Phospholipase D Activity and Phosphatidylinositol 4,5-bisphosphate Synthesis in Clonal {beta} Cells HIT-T15. Mol. Pharmacol. 67(6): p. 2162-2172.
Primary	Neutrophils	Human	BioPORTER Protein Delivery Reagent	Fessler, M.B., Arndt, P.G., Just, I., Nick, J.A., Malcolm, K.C. and Worthen, G.S. (2007) Dual role for RhoA in suppression and induction of cytokines in the human Neutrophil. Blood 109: 1248 - 1256.
Primary	LEC	Mouse	BioPORTER Protein Delivery Reagent	Lofgren, S., Fernando, M.R., Xing, K., Wang, Y., Kuszynski, C.A., Ho, Y. and Lou, M.F. (2008) Effect of Thioltransferase (Glutaredoxin) Deletion on Cellular Sensitivity to Oxidative Stress and Cell Proliferation in Lens Epithelial Cells of Thioltransferase Knockout Mouse. Invest. Ophthalmol. Vis. Sci. 49(10): 4497-4505.
Primary	Polymorphonuclear Leukocytes	Human	BioPORTER Protein Delivery Reagent	McLaughlin, N.J., Banerjee, A., Khan, SY, Lieber, JL, Kelher, MR, Gamboni-Robertson, F., Sheppard, FR, Moore, EE, Mierau, GW, Elzi, DJ and Silliman, CC. (2008) Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180(12): 8192.
Primary	Neutrophils	Human	BioPORTER Protein Delivery Reagent	McLaughlin, N.J.D., Banerjee, A., Kelher, M.R., Gamboni-Robertson, F., Hamiel, C., Sheppard, F.R., Moore, E.E. and Silliman, C.C. (2006) Platelet-Activating Factor-Induced Clathrin-Mediated Endocytosis Requires beta-Arrestin-1 Recruitment and Activation of the p38 MAPK Signalosome at the Plasma Membrane for Actin Bundle Formation. J. Immunol. 176(11): 7039-7050.
Primary	Adventitial Fibroblasts	Rat	BioPORTER Protein Delivery Reagent	Mounir J. Haurani, M. Eugenia Cifuentes, Alexander D. Shepard, and Patrick J. Pagano (2008) Nox4 Oxidase Overexpression Specifically Decreases Endogenous Nox4 mRNA and Inhibits Angiotensin II¿Induced Adventitial Myofibroblast Migration. Hypertension 52: 143 - 149. (Catalase)
Primary	Cardiac Myocytes	Canine	BioPORTER Protein Delivery Reagent	Oda, T., Yano, M., Yamamoto, T., Tokuhisa, T., Okuda, S., Doi, M., Ohkusa, T., Ikeda, Y., Kobayashi, S., Ikemoto, N. and Matsuzaki, M. (2005) Defective Regulation of Interdomain Interactions Within the Ryanodine Receptor Plays a Key Role in the Pathogenesis of Heart Failure. Circulation 111: 3400 - 3410. (Delivery of 36-mer peptide)
Primary	Neutrophils	Human	BioPORTER Protein Delivery Reagent	Pestonjamasp, K.N., Forster, C., Sun, C., Gardiner, E.M., Bohl, B., Weiner, O., Bokoch, G.M. and Glogauer, M. (2006) Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis. Blood. 108: 2814-2820.
Primary	Vascular Smooth Muscle Cells	Human	BioPORTER Protein Delivery Reagent	Quasnichka, H., Slater, S.C., Beeching, C.A., Boehm, M., Sala-Newby, G.B. and George, S.J. (2006) Regulation of Smooth Muscle Cell Proliferation by β-Catenin/T-Cell Factor Signaling Involves Modulation of Cyclin D1 and p21 Expression. Circ. Res.99: 1329 - 1337.
Primary	Ventricular Muscle	Canine	BioPORTER Protein Delivery Reagent	Tateishi, H., Yano, M., Mochizuki, M., Suetomi, T., Ono, M., Xu, X., Uchinoumi, H., Okuda, S., Oda, T., Kobayashi, S., Yamamoto, T., Ikeda, Y., Ohkusa, T., Ikemoto, N. and Matsuzaki, M. (2009) Defective domain-domain interactions within the ryanodine receptor as a critical cause of diastolic Ca2+ leak in failing hearts Cardiovasc Res. 81(3): 536-545.
Primary	Cortical Neurons	Mouse	BioPORTER Protein Delivery Reagent	Yu, S-W, Andrabi, S.A., Wang, H., Kim, N.S., Poirier, G.G., Dawson, T.M. and Dawson, V.L. (2006) Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death. Proc. Natl. Acad. Sci 103: 18314 - 18319.
Primary	Astroglia	Rat	BioPORTER Protein Delivery Reagent	Zassler, B., Blasig, IE and Humpel, C. (2005) Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells. J Neurooncol¿ 71(2): p. 127.
Primary	Capillary Endothelial Cells	Rat	BioPORTER Protein Delivery Reagent	Zassler, B., Blasig, IE and Humpel, C. (2005) Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells. J Neurooncol¿ 71(2): p. 127.
Primary AML	Acute Myelocytic Leukemia	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem. 276: 35103 - 35110.
Primary Bronchial Epithelial Cells	Bronchial Epithelium	Human	BioPORTER Protein Delivery Reagent	Kemp, P.A., Sugar, R.A. and Jackson A.D. (2004) Nucleotide-Mediated Mucin Secretion from Differentiated Human Bronchial Epithelial Cells. Am. J. Respir. Cell Mol. Biol. 31(4): 446-455.
Primary CML	Chronic Myelocytic Leukemia	Human	BioPORTER Protein Delivery Reagent	Zelphati, O., Wang, Y., Kitada, S., Reed, J.C., Felgner, P.L., and Corbeil, J. (2001) Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System. J. Biol. Chem 276: 35103 - 35110.
Primary Cortical Neurons	Primary Cortical Neurons	Mouse	BioPORTER Protein Delivery Reagent	Wang, H., Yu, S-W, Koh, D.W., Lew, J., Coombs, C. Bowers, W., Federoff, H.J., Poirier, G.G., Dawson, T.M. and Dawson, V.L. (2004) Apoptosis-Inducing Factor Substitutes for Caspase Executioners in NMDA-Triggered Excitotoxic Neuronal Death. J. Neurosci. 24 (48): 10963-10973.
Primary Eye Lens Epithelium	Eye Lens	Rat	BioPORTER Protein Delivery Reagent	Boyle, D.L., Carman, P. and Takemoto, L. (2002) Translocation of macromolecules into whole rat lenses in culture. Mol. Vis. 8: 226-34.
Primary Eye Lens Epithelium	Eye Lens	Rat	BioPORTER Protein Delivery Reagent	Marques, C., Pereira, P., Taylor, A., Liang, J.N., Reddy, V.N., Szweda, L.I. and Shang, F. (2004) Ubiquitin-dependent lysosomal degradation of the HNE-modified proteins in lens epithelial cells. FASEB J. published 9 July 2004, 10.1096/fj.04-1743fje.
Primary Microvascular Endothelial Cells	Neonatal Dermis	Human	BioPORTER Protein Delivery Reagent	Mammoto, A., Huang, S., Moore, K., Oh, P. and Ingber, D.E. (2004) Role of RhoA, mDia, and ROCK in Cell Shape-dependent Control of the Skp2-p27kip1 Pathway and the G1/S Transition. J. Biol. Chem. 279(25): p. 26323-26330.
Primary Neutrophils	Venous Blood	Human	BioPORTER Protein Delivery Reagent	Diebold, B.A., Fowler, B., Lu, J., Dinauer, M.C. and Bokoch, G.M. (2004)¿Antagonistic Cross-talk between Rac and Cdc42 GTPases Regulates Generation of Reactive Oxygen Species. J. Biol. Chem. 279(27): p. 28136-28142.
Primary Neutrophils	Venous Blood	Human	BioPORTER Protein Delivery Reagent	Gomez-Cambronero, J., Horn, J., Paul, C.C. and Baumann, M.A. (2003) Granulocyte-Macrophage Colony-Stimulating Factor Is a Chemoattractant Cytokine for Human Neutrophils: Involvement of the Ribosomal p70 S6 Kinase Signaling Pathway. J. Immunol. 171: 6846-6855.
Primary Neutrophils	Venous Blood	Human	BioPORTER Protein Delivery Reagent	Rane, M.J., Pan, Y., Singh, S., Powell, D.W., Wu, R., Cummins, T., Chen, Q., McLeish, K.R., and Klein, J.B. (2003) Heat Shock Protein 27 Controls Apoptosis by Regulating Akt Activation J. Biol. Chem. 278: 27828 - 27835.
Primary Neutrophils	Venous Blood	Human	BioPORTER Protein Delivery Reagent	Zhao, T., Benard, V., Bohl, B.P. and Bokoch, G.M. (2003) The molecular basis for adhesion-mediated suppression of reactive oxygen species generation by human neutrophils. J. Clin. Invest. 112: 1732 - 1740.
Primary Retina	Chicken E8 Retina	Chicken	BioPORTER Protein Delivery Reagent	Xu, G., Craig, A.W.B., Greer, P., Miller, M., Anastasiadis, P.Z., Lilien, J. and Balsamo, J. (2004) Continuous association of cadherin with {beta}-catenin requires the non-receptor tyrosine-kinase Fer. J. Cell Sci. 117(15): p. 3207-3219.
Primary Retinal Growth Cone	Retinal Growth Cone	Mouse	BioPORTER Protein Delivery Reagent	Suh, L.H.,. Oster, S.F., Soehrman, S.S., Grenningloh, G.and Sretavan, D.W. (2004) L1/Laminin Modulation of Growth Cone Response to EphBTriggers Growth Pauses and Regulates the Microtubule Destabilizing Protein SCG10. J. Neurosci. 24 (8) 1976-1986.
rBCEC4	Brain Capillary Endothelial	Rat	BioPORTER Protein Delivery Reagent	Zassler, B., Blasig, IE and Humpel, C. (2005) Protein delivery of caspase-3 induces cell death in malignant C6 glioma, primary astrocytes and immortalized and primary brain capillary endothelial cells. J Neurooncol.¿71(2): p. 127.
SH-SY5Y	Neuroblastoma	Human	BioPORTER Protein Delivery Reagent	Yuste, V.J., Sanchez-Lopez, I., Sole, C., Moubarak, R.S.,¿Bayascas, J.R., Dolcet, X., Encinas, M., Susin, S.A. and¿Comella, J.X. (2005)¿The Contribution of Apoptosis-inducing Factor, Caspase-activated DNase, and Inhibitor of Caspase-activated DNase to the Nuclear Phenotype and DNA Degradation during Apoptosis. J. Biol. Chem. 280(42): p. 35670-35683. (Antibody Delivery).
SH-SY5Y, pgsA-745	bone marrow neuroblast	Human	BioPORTER Protein Delivery Reagent	Claire H. Michel, Satish Kumar, Dorothea Pinotsi, Alan Tunnacliffe, Peter St., George-Hyslop, Eckhard Mandelkow,Eva-Maria Mandelkow, Clemens F. Kaminski and Gabriele S. Kaminski Schierle. Extracellular Monomeric Tau Protein Is Sufficient to Initiate the Spread of Tau Protein Pathology. J. Biol. Chem. 2014, 289:956-967.
UM-SCC-23	Squamous Cell Carcinoma	Human	BioPORTER Protein Delivery Reagent	Shao, H., Cheng, HY, Cook, R.G. and Tweardy, DJ (2003) Identification and Characterization of Signal Transducer and Activator of Transcription 3 Recruitment Sites within the Epidermal Growth Factor Receptor. Cancer Res 63: 3923.

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