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Qiagen/PAXgene Blood miRNA Kit/For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes/763134
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PAXgene Blood miRNA Kit
Print
For purification of miRNA and total RNA from whole blood
- Integrated system for collection, stabilization, and purification
- Effective purification of miRNA and total RNA
- RNA stabilization for up to 3 days at 18–25°C
- Stabilization for at least 50 months at –20°C or –70°C
The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.
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Cat No./ID:763134 PAXgene Blood miRNA Kit (50) $717.00 For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes |
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Blood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours, at 2–8°C for up to 5 days, or at –20°C or –70°C for at least 50 months without showing any significant RNA degradation or changes in transcript levels. Total RNA >18 nucleotides is purified from the stabilized blood samples with the PAXgene Blood miRNA Kit and includes both mRNA and small RNAs such as miRNA (see figure "High yields of small RNA species"). The miRNA species can be analyzed using the QIAGEN miScript PCR System (see figure "Analysis of miRNA species") or other real-time RT-PCR systems.
Principle
Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn.
Procedure
With the PAXgene Blood miRNA Kit, total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. Purification can be carried out in a manual format or it is automatable on the QIAcube (see figure "The QIAcube").
Applications
The purified total RNA is ready to use and is ideally suited for any downstream application, including:
- RT-PCR and real-time RT-PCR
- Differential display
- cDNA synthesis
- Northern, dot, and slot blot analyses
- Primer extension
- Poly A+ RNA selection
- RNase/S1 nuclease protection
- Microarray analysis
Specifications
Features | Specifications |
| Applications | RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis |
| Elution volume | 40 µl |
| Format | Spin column |
| Main sample type | Whole blood |
| Processing | Manual (centrifugation) or on QIAcube |
| Sample amount | 2.5 ml |
| Technology | Silica technology |
| Time per run or per prep | 90 minutes/12 samples |
| Yield | >3 µg (total RNA) |
Product Resources
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