QIAamp Virus BioRobot MDx Kit
- Rapid isolation of high-quality, ready-to-use viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
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Cat No./ID:965652 QIAamp Virus BioRobot MDx Kit (12) $4,773.00 For 12 x 96 preps for use on the BioRobot MDx; includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNA |
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Product Details
The QIAamp Virus BioRobot MDx process provides uniform DNA and RNArecovery and purity across the 96-well plate with no detectablecross-contamination between samples. Up to 265µl of cell-free body fluids can be processed. The typical elution volumeis75 µl. Viruses can be detected with high sensitivity when using this kit, which is illustrated by hit-rate studies (see table). Further advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids. The QIAamp Virus BioRobot MDx provides reliable results in downstream applications.
| PCR product (n=96) | Instrument 1 | Instrument 2 | ||||
| Mean CT | Standard deviation | %CV | Mean CT | Standard deviation | %CV | |
|---|---|---|---|---|---|---|
| Mimic virus | 28.26 | 0.10 | 0.35 | 28.38 | 0.18 | 0.65 |
| Internal control | 31.98 | 0.16 | 0.51 | 31.87 | 0.22 | 0.70 |
The QIAamp Virus BioRobot MDx Kit simplifies isolation of viral RNA and DNA from cell-free body fluids with a fast automated 96-well–plate procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures.
The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. Advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids for laboratories. QIAamp sample preparation technology is fully licensed.
The QIAamp Virus BioRobot MDx Kit provides automated, high-throughput purification of viral RNA and DNA on the BioRobot MDx using proven QIAamp technology. The 96-well plates provide the flexibility of processing 32–96 samples, in increments of 8. Sample sources include:
- Plasma and serum
- CSF
- Other cell-free body fluids
- Cell-culture supernatants
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time PCR |
| Elution volume | 75 µl |
| For automated processing | BioRobot MDx Workstation |
| Format | 96-well plates |
| Main sample type | Serum, plasma |
| Processing | Automated |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral DNA, viral RNA |
| Sample amount | 265 µl |
| Samples per run (throughput) | 36-92 samples per run |
| Technology | Silica technology |
| Time per run or per prep | <2.5> |
| Yield | >90% recovery |
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