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Qiagen/PAXgene Bone Marrow RNA Tubes/50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit/764114

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Qiagen/PAXgene Bone Marrow RNA Tubes/50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit/764114

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Qiagen

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764114

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Sample Collection & Stabilization
DNA5
RNA19
Tissue/FFPE5

PAXgene Bone Marrow RNA Tubes

For collection of bone marrow samples and stabilization/purification of intracellular RNA

Integrated system for storage, stabilization, and purification
Immediate stabilization of intracellular RNA at collection
Rapid purification from heterogeneous bone marrow samples
Safe and easy transportation

The PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C.

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Cat No./ID:764114

PAXgene Bone Marrow RNA Tubes (50)

$1,139.00

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50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit

For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

Storage under different conditions.

Bone marrow samples were stored in PAXgene Bone Marrow RNA Tubes [A] for 3 days at 18–25°C, [B] for 5 days at 2–8°C, or [C] subjected to 2 freeze–thaw cycles. RNA was purified using the PAXgene Bone Marrow RNA Kit, and RNA purity was analyzed by absorbance. The A₂₆₀/A₂₈₀ ratios indicate highly pure RNA from samples stored under different conditions.

RNA stability at room temperature.

Bone marrow samples were collected from 39 individuals and immediately transferred to [A] PAXgene Bone Marrow RNA Tubes or [B] EDTA tubes. RNA was purified using the PAXgene Bone Marrow RNA Kit or a reference silica-membrane-based method at day 0 or after 2 days storage at room temperature. Expression levels of 5 marker transcripts for each sample (IL-8, p53, cox-2, IL-1β, c-fos) were quantified using real-time RT-PCR. The high correlation of C_T values (threshold cycles) for the samples stabilized in PAXgene Bone Marrow RNA Tubes indicates that the levels of these transcripts did not significantly change during storage, in contrast to the samples stored in EDTA tubes.

RNA yields from bone marrow samples.

Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit. Yields are indicated for 295 samples, from both in-house and external studies. The median yield for the 295 samples was 11.0 μg RNA per 2 ml sample. Note that RNA yields can vary greatly due to the extreme heterogeneity of bone marrow samples.

High RNA integrity after freeze–thaw cycles.

Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit after the indicated number of freeze–thaw cycles. The RIN values did not change significantly, indicating that RNA remained intact in the samples up to 2 freeze–thaw cycles.

RNA stabilized at 2–8°C.

Bone marrow samples were collected in duplicate and stabilized in PAXgene Bone Marrow RNA Tubes. RNA was purified from the samples using the PAXgene Bone Marrow RNA Kit with no storage or after 5 days storage at 2–8°C. RNA integrity was evaluated using the RNA integrity numbers (RIN), measured on the Agilent 2100 Bioanalyzer. Results are shown for 3 representative donors. The RIN did not change significantly during storage, indicating that the RNA remains stable in the sample.

Stability of 5 transcripts at room temperature.

The changes in mRNA levels (ΔC_T) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.

Performance

The PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure "RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures "RNA stability at room temperature", "RNA stabilized at 2–8°C", and "High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis byminimizing ex vivo changes in gene expression (see figure "Stability of 5 transcripts at room temperature").An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A₂₆₀/A₂₈₀ values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure "Storage under different conditions").

Principle

Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.

Procedure

Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.

Applications

RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including

Real-time RT-PCR analysis of specific transcripts
Array analysis of complex expression profiles

Specifications

Features	Specifications
Additive/concentration	Additive 11.1 ml
Closure type/color	BD Hemogard/Red
Draw volume	2 ml
Label type	Paper
Quantity	50/box
Tube size	16 x 125 mm

Product Resources

Brochures & Guides (3)

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	All insights start with the sample: Your comprehensive guide for isolating top-quality RNA	Show details
	(EN) - Analyzing Gene Expression and Regulation	Show details
	(EN) - PAXgene Bone Marrow RNA System Brochure For collection, storage, and transport of bone marrow samples and stabilization and purification of intracellular RNA	Show details

FAQs (17)

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	Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples? FAQ ID -2501	View
	Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin? FAQ ID -2502	View
	Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube? FAQ ID -2503	View
	How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube? FAQ ID -2504	View
	How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long? FAQ ID -2505	View
	What RNA yields are expected with the PAXgene Bone Marrow RNA System? FAQ ID -2506	View
	Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA? FAQ ID -2507	View
	Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation? FAQ ID -2508	View
	Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube? FAQ ID -2509	View
	Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System? FAQ ID -2510	View
	What is in the elution buffer in the PAXgene Bone Marrow RNA kit? FAQ ID -2511	View
	Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit? FAQ ID -2512	View
	I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation? FAQ ID -2513	View
	Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System? FAQ ID -2514	View
	Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach? FAQ ID -2515	View
	How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed? FAQ ID -2516	View
	Where can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515	View

Kit Handbooks (2)

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	(EN) - PAXgene Bone Marrow RNA Kit Handbook For isolation and purification of intracellular RNA from whole bonemarrow samples stabilized in PAXgene Bone Marrow RNA Tubes	Show details
	(EN) - PAXgene Bone Marrow Tube product circular For the collection of human bone marrow and immediate stabilization of intracellular RNA	Show details

Scientific Posters (2)

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	(EN) - Evaluation of the PAXgene Bone Marrow RNA System for Stabilization and Purification of Cellular RNA Guenther et al., AMP 2006	Show details
	(EN) - RNA Stabilization of Human Whole Bone Marrow Aspirates for High Reliability of Gene Expression Analysis Guenther et al., AACR 2007	Show details

Safety Data Sheets (1)

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	MSDS PAXgene Bone Marrow RNA Tubes (50)

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