RNeasy Protect Cell Mini Kit
- Immediate RNA stabilization in sorted or cultured cells
- No need to remove medium prior to cell stabilization
- Storage and shipping of cells without dry ice
- Archiving of cells at –20ºC or –80ºC
- Purification of high-quality RNA in minutes
The RNeasy Protect Cell Mini Kit includes RNAprotect Cell Reagent, which stabilizes RNA and DNA in sorted or cultured cells and allows convenient and reliable transport of the cells at ambient temperature. The stabilized cells can be stored or shipped at 30°C (1 day), 15–25°C (7 days), or 2–8°C (4 weeks). The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. Stabilized cells are lysed and spun through gDNA Eliminator spin columns to effectively remove genomic DNA. High-quality RNA is then purified using RNeasy spin columns. The purification procedure can be automated on the QIAcube.
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Cat No./ID:74624 RNeasy Protect Cell Mini Kit (50) $391.00 RNAprotect Cell Reagent (50 ml) and RNeasy Plus Mini Kit (50) |
Product Details
The RNeasy Protect Cell Mini Kit is ideal for gene expression analysis experiments where cells need to be delivered from one lab to another (see Table 1).
| Assay result | RNAprotect Cell Reagent | RNAprotect TissueReagent |
|---|---|---|
| RNA yield using RNeasy technology (µg) | 10 | 3.5 |
| Real-time RT-PCR of abl (CT value) | 25 | 26 |
Cells in RNAprotect Cell Reagent can be stored or transported for up to 1 day at 30ºC, up to 7 days at room temperature (15–25ºC), or up to 4 weeks at 2–8ºC, and can also be stored long-term at –20ºC or –80ºC (see Table 2, and figures "Effective inhibition of PMA induction" and "More reliable quantification of CD83 transcript").
| Cell storage conditions | RIN value of purified RNA* |
|---|---|
| 30ºC (1 day) | 9.8 |
| 25ºC (7 days) | 9.7 |
| 2–8ºC (28 days) | 10 |
| –20ºC (28 days) | 9.8 |
| –80ºC (28 days) | 9.8 |
The RNeasy Protect Cell Mini Kit provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy technology, combined with the RNA-stabilizing properties of RNAprotect Cell Reagent, allows purification of high-quality, intact RNA. Sorted or cultured cells are mixed with RNAprotect Cell Reagent, which immediately stabilizes the cellular RNA, ensuring reliable results in downstream gene expression analysis. RNAprotect Cell Reagent can be added directly to cells in culture medium or buffer, eliminating the need to wash or process cells (e.g., by trypsin treatment). Alternatively, RNAprotect Cell Reagent can be added to pelleted cells. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification.
Cells in RNAprotect Cell Reagent can be transported at ambient temperature, avoiding the need to freeze cell pellets in liquid nitrogen and to ship the frozen pellets on dry ice. RNA purified using the RNeasy Plus Mini Kit is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as:
- Quantitative real-time RT-PCR
Specifications
Features | Specifications |
| Applications | PCR, qPCR, real-time RT-PCR, microarray |
| Elution volume | 30–50 µl |
| Format | Spin column |
| Main sample type | Cultured cells |
| Processing | Manual (centrifugation) |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Total RNA |
| Sample amount | Max. 10e7 cells |
| Stabilization | Yes |
| Technology | Silica technology |
| Yield | Varies |
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