Ni-NTA Agarose
For purification of His-tagged proteins by gravity-flow chromatography
- One-step purification from crude lysate to >95% pure protein
- High binding affinity and high capacity
- Choice of purification under native or denaturing conditions
- Precharged, ready-to-use matrices for any scale of purification
- Automated purification and assay protocols
Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix. After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.
- Buy Products
- Product Details
- Specifications
Buy Products
Cat No./ID:30210 Ni-NTA Agarose (25 ml) $323.00 25 ml nickel-charged resin (max. pressure: 2.8 psi) |
Cat No./ID:30230 Ni-NTA Agarose (100 ml) $1,114.00 100 ml nickel-charged resin (max. pressure: 2.8 psi) |
Cat No./ID:30250 Ni-NTA Agarose (500 ml) $4,818.00 500 ml nickel-charged resin (max. pressure: 2.8 psi) |
Product Details
Ni-NTA Agarose provides Ni-NTA coupled to a Sepharose CL-6B support and offers high binding capacity and minimal nonspecific binding (see figure One-step purification under native conditions). This material has excellent handling properties for most scales of batch and column purification. Ni-NTA Agarose is available separately or as a component of QIAexpress Kits, which are complete kits for efficient expression and purification of His-tagged proteins from E. coli .
The QIAexpress Ni-NTA Protein Purification System is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins which contain an affinity tag of six or more histidine residues (consecutive or alternating) — the His tag. This technology allows one-step purification of almost any His-tagged protein from any expression system under native or denaturing conditions. NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching and results in a greater binding capacity and protein preparations with higher purity than those obtained using other metal-chelating purification systems. The QIAexpress system can be used to purify His-tagged proteins from any expression system, including baculovirus, mammalian cells, yeast, and bacteria.
| Denaturants | Detergents | Reducing agents | Others | Salts | For long-term storage |
|---|---|---|---|---|---|
| 6 M Gu·HCl | 2% Triton X-100 | 20 mM β-ME | 50% Glycerol | 4 M MgCl2 | Up to 30% ethanol |
| 8 M Urea | 2% Tween 20 | 10 mM DTT | 20% Ethanol | 5 mM CaCl2 | or 100 mM NaOH |
| 1% CHAPS | 20 mM TCEP | 20 mM Imidazole | 2 M NaCl |
Ni-NTA matrices provide reliable, one-step purification of His-tagged proteins suitable for any application, including:
- Structural and functional investigations
- Crystallization for determination of three-dimensional structure
- Protein–protein and protein–DNA interaction assays
- Immunization to produce antibodies
- Scaling up purification to production scale
Specifications
Features | Specifications |
| Applications | Proteomics |
| Bead size | 45–165 µm |
| Binding capacity | Up to 50 mg/ml |
| FPLC | No |
| Gravity flow or spin column | Gravity flow |
| Processing | Manual/Automated |
| Scale | Large scale |
| Special feature | Batch and column purification |
| Start material | Cell lysate |
| Support/matrix | Sepharose CL-6B |
| Tag | 6xHis tag |
| Yield | 100 µg – 100 mg |
Product Resources
Customers who bought these products also bought
- Cat No./ID:34964
Polypropylene Columns (5 ml)
50/pack, 5 ml capacity$189.00Add To Cart - Cat No./ID:34924
Polypropylene Columns (1 ml)
50/pack, 1 ml capacity$147.00Add To Cart - Cat No./ID:330519
RT² SYBR Green Fluor qPCR Mastermix (25ml)
Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactionsor 5000 x 10 µl reactions$1,577.00Add To Cart - Cat No./ID:12143
QIAGEN Plasmid Midi Kit (25)
25 QIAGEN-tip 100, Reagents, Buffers$303.00Add To Cart - Cat No./ID:28704
QIAquick Gel Extraction Kit (50)
For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$124.00Add To Cart - Cat No./ID:27106X4
QIAprep Spin Miniprep Kit (1000)
For 1000 high-purity plasmid minipreps: 1000 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$1,360.00Add To Cart - Cat No./ID:27104
QIAprep Spin Miniprep Kit (50)
For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$96.90Add To Cart - Cat No./ID:28104
QIAquick PCR Purification Kit (50)
For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$123.00Add To Cart - Cat No./ID:28706X4
QIAquick Gel Extraction Kit (1000)
For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$1,879.00Add To Cart - Cat No./ID:28604
MinElute Gel Extraction Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2ml)$141.00Add To Cart - Cat No./ID:79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart - Cat No./ID:12145
QIAGEN Plasmid Midi Kit (100)
100 QIAGEN-tip 100, Reagents, Buffers$1,133.00Add To Cart - Cat No./ID:76104
RNAprotect Tissue Reagent (50ml)
50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples
$88.60Add To Cart - Cat No./ID:28106
QIAquick PCR Purification Kit (250)
For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$561.00Add To Cart - Cat No./ID:27106
QIAprep Spin Miniprep Kit (250)
For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$422.00Add To Cart - Cat No./ID:77023
exoRNeasy Serum/Plasma Starter Kit (20)
For purification of total exosome-derived RNA from serum/plasma;includes 20 exoEasy columns (10 Maxi, 10 Midi) and 20 RNeasy MinElute spin columns$650.00Add To Cart - Cat No./ID:12362
EndoFree Plasmid Maxi Kit (10)
10 QIAGEN-tip 500, Reagents, 10 QIAfilter Maxi Cartridges, Endotoxin-free Buffers$355.00Add To Cart - Cat No./ID:30761
Ni-NTA Superflow Cartridges (5 x 5 ml)
5 cartridges pre-filled with 5 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems$644.00Add To Cart - Cat No./ID:28004
MinElute PCR Purification Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2ml)$141.00Add To Cart - Cat No./ID:28006X4
MinElute PCR Purification Kit (1000)
1000 MinElute Spin Columns, Buffers, Collection Tubes (2ml)Add To Cart
ebiomall.com
