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Qiagen/Ni-NTA Superflow/25 ml nickel-charged resin (max. pressure: 140 psi)/30410
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Qiagen/Ni-NTA Superflow/25 ml nickel-charged resin (max. pressure: 140 psi)/30410
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Qiagen
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30410
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Ni-NTA Superflow

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For purification of 6xHis-tagged proteins by FPLC

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Cat No./ID:30410
Ni-NTA Superflow (25 ml)
$410.00
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25 ml nickel-charged resin (max. pressure: 140 psi)
Cat No./ID:30430
Ni-NTA Superflow (100 ml)
$1,377.00
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100 ml nickel-charged resin (max. pressure: 140 psi)
Cat No./ID:30450
Ni-NTA Superflow (500 ml)
$5,603.00
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500 ml nickel-charged resin (max. pressure: 140 psi)
The Ni-NTA Superflow is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Specifications

Features
Specifications
ApplicationsProteomics
Bead size60–160 µm
Binding capacityUp to 50 mg/ml
FPLCYes
Gravity flow or spin columnGravity flow or automated
Number of preps per run1–24 samples per run
ProcessingManual
ScaleLarge scale
Special featureBatch and column purification
Start materialCell lysate
Support/matrixSuperflow
Tag6xHis tag
YieldDepends on binding capacity

Product Resources

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FAQs (16)
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What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
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How can I remove imidazole from a protein sample?
FAQ ID -91
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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
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Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
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What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
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What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
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How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
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Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
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Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
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Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
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What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
View
How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
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Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
View
Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
View
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
View
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
View
Kit Handbooks (2)
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The QIAexpressionist - (EN)
A handbook for high-level expression and purification of 6xHis-tagged proteins
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(EN) - Important Note for Ni-NTA Users
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Technical Information (3)
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Complete solutions for membrane protein analysis - (EN)
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Critical factors for successful protein crystallization - (EN)
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Reliable purification of GST-, His-, and Strep-tagged proteins - (EN)
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Scientific Posters (3)
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(EN) - New Ni-NTA Cartridges — the faster way to purer proteins
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(EN) - Novel cell-free expression system for synthesis of proteins used in structural analyses
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(EN) - Process Development: Scaling Up Human IL-1b Production, Tag Removal, and X-Ray Crystallography
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Quick-Start Protocols (2)
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Automated Purification of 6xHis-tagged Proteins from E. coli Using Ni-NTA Superflow under Native Conditions (EN)
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Ni-NTA Agarose Purification of 6xHis-tagged Proteins from E. coli under Native Conditions (EN)
Show details
Safety Data Sheets (4)
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MSDS Ni-NTA Superflow (100 ml)
MSDS Ni-NTA Superflow (25 ml)
MSDS Ni-NTA Superflow (500 ml)
CofA
References
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