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Penta·His Antibody, BSA-free
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For sensitive detection of His-tagged proteins
- Detection of C-terminal, N-terminal, and internal His tags
- Highly sensitive and specific detection of HHHHH epitopes
Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.
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Cat No./ID:34660 Penta·His Antibody, BSA-free (100 μg) $842.00 100 μg mouse anti-(H)5 (lyophilized, BSA-free, for 1000 ml working solution) |
The Penta·His Antibody, BSA-freeis intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
| Property | Value |
|---|---|
| Detection: | N-terminal, C-terminal, and internal 6xHis tags |
| Sensitivity in dot blots:* | 0.5 ng |
| Isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Lyophilized |
| Working solution: | 1000 ml |
Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)
| Protein | Tag location | Sequence |
|---|---|---|
| A DHFR | N-terminus | MRGSHHHHHHGSVRPLNCIV... |
| B DHFR | C-terminus | ...VYEKKDGSRSHHHHHH |
| C Thioredoxin | N-terminus | MRGSHHHHHHGSSDKIIHLT... |
| D TNFα | N-terminus | MRHHHHHHQSTESMIRDV... |
| E TFIIAγ | N-terminus | MGSSHHHHHHSSGLVPRGSH... |
| F Chaperonin | C-terminus | ...AKIAKDGSRSHHHHHH |
| G DNA Polymerase | C-terminus | ...EDWLSAKERSHHHHHH |
| H TFIIAαβ | Internal | ...QQQHQPQQQQHHHHHHQQAQPQQTVP... |
For other experiments with Anti·His Antibodies see figures Identification of positive expression clones by colony blotting and His-tagged thioredoxin detected by Penta·His Antibodies in yeast cells
Principle
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.
Applications
QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including:
- Colony, dot, and western blotting procedures
- Screening for positive expression clones
- Monitoring expression levels and stability of His-tagged proteins
- Immunoprecipitation and ELISA
Specifications
Features | Specifications |
| Detection | Secondary antibody required |
| Epitope detected | HHHHH |
| Sensitivity in dot blots (chemiluminescent detection) | 10 pg |
| Sensitivity in dot blots (chromogenic detection) | 0.5 ng |
| Sensitivity in Western blots (chemiluminescent detection) | 50 pg |
| Sensitivity in Western blots (chromogenic detection) | 2 ng |
| Substrate for blot detection | Dependent on secondary antibody |
| Substrates for assay procedure | Dependent on secondary antibody |
| Tag | His-tag |
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