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Penta·His Alexa Fluor 647 Conjugate
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For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 647nm
- Highly specific and sensitive detection of His-tagged proteins
- Direct detection without the need for a secondary antibody
- Broad instrument compatibility and high photostability
Penta·His Alexa Fluor 647 Conjugate is for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive histidine residues) at 647 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies, and fluorescence-activated cell sorting (FACS).
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Cat No./ID:35370 Penta·His Alexa Fluor 647 Conjugate $255.00 125 μl Penta·His Alexa Fluor 647 Conjugate, 200 μg/ml |
The Penta·His Alexa Fluor 647 Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
| Property | Value |
|---|---|
| Detection: | Five consecutive histidine residues |
| Antibody isotype: | Mouse IgG1 |
| Cross-reactivity: | Negligible with crude E. coli, yeast, mammalian, or insect cell lysates |
| Form: | Stabilized stock solution |
| Working solution: | Dependent on application |
Principle
Penta·His Antibodies are available as Alexa Fluor 488, 532, 555, and 647 conjugates, giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum.
Procedure
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Applications
The Penta·His Alexa Fluor 647 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization, and targeting studies
Specifications
Features | Specifications |
| Detection | Direct |
| Epitope detected | HHHHH |
| Tag | His tag |
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