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Qiagen/QIAamp MinElute Virus Spin Kit/For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)/57704
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Qiagen/QIAamp MinElute Virus Spin Kit/For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)/57704
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57704
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QIAamp MinElute Virus Spin Kit

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For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids

  • Rapid purification of high-quality viral DNA and RNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on theQIAcube Connect.

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Cat No./ID:57704
QIAamp MinElute Virus Spin Kit (50)
$295.00
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For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml)
The QIAamp MinElute Virus Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

9
High sensitivity in RT-PCR.
9
High sensitivity in PCR.
The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml.
9
QIAamp MinElute Virus Spin procedure.
Performance

Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").

Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:

  • PCR and quantitative real-time PCR
  • Infectious disease research
Principle

The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures.

The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.

Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications

The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:

  • Fresh or frozen plasma
  • Serum
  • Other cell-free body fluids

Specifications

Features
Specifications
ApplicationsPCR, real-time PCR
Elution volume20–150 µl
FormatMinElute columns
Main sample typeSerum, plasma
ProcessingManual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Sample amount200 µl
TechnologySilica technology
Time per run or per prep<1>
YieldVaries

Product Resources

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Brochures & Guides (2)
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QIAamp virus kits - (EN)
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Product Profile - QIAamp® virus kits
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FAQs (9)
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How do I safely inactivate biohazardous flow-through material?
FAQ ID -12
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What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
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Is QIAGEN carrier RNA sold separately?
FAQ ID -351
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them?AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
View
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
View
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
View
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
View
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
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Kit Handbooks (1)
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QIAamp MinElute Virus Spin Handbook
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Safety Data Sheets (2)
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MSDS QIAamp MinElute Virus Spin Kit (50)
CofA
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