HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
- High PCR specificity without the need for optimization
- Easy reaction setup at room temperature
- Ready-to-use master mix format reduces pipetting steps
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- Product Details
- Specifications
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Cat No./ID:203443 HotStarTaq Master Mix Kit (250 U) $227.00 3 x 0.85 ml HotStarTaq Master Mix (contains 250 units HotStarTaq DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP)and 2 x 1.7 ml RNase-Free Water |
Cat No./ID:203445 HotStarTaq Master Mix Kit (1000 U) $830.00 12 x 0.85 ml HotStarTaq Master Mix (contains 1000 units HotStarTaq DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP) and 8 x 1.7 ml RNase-Free Water |
Cat No./ID:203446 Hot StarTaq Master Mix Kit (2500 U) $1,871.00 1 x 25 ml HotStarTaq Master Mix (contains 2500 units HotStarTaq DNA Polymerase PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP) and 1 x 50 ml RNase-Free Water |
Product Details
Each lot of HotStarTaq Master Mix Kit is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified. HotStarTaq Master Mix Kit outperformed kits tested from other suppliers and ensures high specificity and superior performance in hot-start PCR (see figures "Higher specificity with different primer–template systems" and "Superior performance " and table). The innovative PCR buffer provided with the kit ensures specificity over a wide range of PCR conditions, minimizing the need for optimization (see figure "Tolerance to variable temperatures and magnesium concentrations").
The combination of high specificity and easy handling makes the HotStarTaq Master Mix Kitsuitable for use with complex genomic or cDNA templates (see figure "Effect of hot start on RT-PCR performance"), multiple primer pairs (see figure "Specific amplification in multiplex PCR"), and templates isolated from difficult sources or very low-copy targets (see figure "Highly sensitive single-cell PCR"). It is also suitable forprojects such as genetic screening, in which large numbers of samples are amplified.
| HotStarTaq DNA Polymerase | Hot-start enzyme from Supplier AII | Antibody-mediated | Manual | Wax barrier | |
|---|---|---|---|---|---|
| Specific amplification | ++ | + | + | +/– | +/– |
| Minimal PCR optimization | ++ | +/– | +/– | – | – |
| Easy to use | ++ | ++ | + | – | – |
HotStarTaq DNA Polymerase specifications
Concentration: 5 units/µlRecombinant enzyme: YesSubstrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTPExtension rate: 2–4 kb/min at 72°CHalf-life: 10 min at 97°C ; 60 min at 94°CAmplification efficiency: ≥105 fold5"–>3" exonuclease activity: YesExtra A addition: Yes3"–>5" exonuclease activity: NoContaminating nucleases: NoContaminating RNases: NoContaminating proteases: NoSelf-priming activity: No
HotStarTaq Master Mix is a ready-to-use mixture of HotStarTaq DNA Polymerase, QIAGEN PCR Buffer, and dNTPs. HotStarTaq DNA Polymerase, a modified form of Taq DNA Polymerase, provides high specificity in hot-start PCR.
HotStarTaq DNA Polymerase
HotStarTaq DNA Polymerase is supplied in an inactive state and has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during PCR setup and the initial PCR cycle (see figures "Superior performance in hot-start PCR" and "Higher specificity with different primer–template systems"). HotStarTaq DNA Polymerase is activated by a 15-minute incubation at 95°C, which can be incorporated into any existing thermal-cycler program.
QIAGEN PCR Buffer
QIAGEN PCR Buffer maintains specific amplification in every cycle of PCR by promoting a high ratio of specific-to-nonspecific primer binding during the annealing step in each PCR cycle (see figure "Increased specificity of primer annealing"). Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the buffer provides stringent primer-annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is therefore often minimal or not required (see figures "Wide annealing temperature window" and "Tolerance to variable magnesium concentration").
HotStarTaq Master Mix Kit is highly suitable for a wide variety of applications, including challenging applications such as amplification of:
- Complex genomic templates
- Complex cDNA templates (e.g., RT-PCR)
- Very low-copy targets (e.g., single-cell PCR)
- Reactions with multiple primer pairs
Specifications
Features | Specifications |
| Applications | PCR, RT-PCR, Complex genomic templates, very low-copy targets |
| Enzyme activity | 5"-> 3" exonuclease activity |
| Mastermix | Yes |
| Reaction type | PCR amplification |
| Real-time or endpoint | Endpoint |
| Sample/target type | Genomic DNA and cDNA |
| Single or multiplex | Single |
| With/without hotstart | With hotstart |
Product Resources
Product citations in scientific articles
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