HotStarTaq Plus DNA Polymerase
- High PCR specificity with minimal optimization
- Fast 5-minute enzyme activation time
- Ready-to-load PCR buffer for faster and easier handling
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- Product Details
- Specifications
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Cat No./ID:203603 HotStarTaq Plus DNA Polymerase (250) $167.00 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 |
Cat No./ID:203605 HotStarTaq Plus DNA Polymerase (1000) $593.00 1000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 |
Cat No./ID:203607 HotStarTaq Plus DNA Polymerase (5000) $2,658.00 5000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 |
Cat No./ID:203609 HotStarTaq Plus DNA Polymerase (25000) $11,983.00 25,000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2 |
Product Details
| HotStarTaq Plus DNA Polymerase | HotStarTaq DNA Polymerase | Hot-start enzyme from Supplier AII | Supplier R | Supplier I (antibody-mediated) | Manual | Wax barrier | |
|---|---|---|---|---|---|---|---|
| Specific amplification | ++ | ++ | + | ++ | + | +/– | +/– |
| Minimal PCR optimization | ++ | ++ | +/– | +/– | +/– | – | – |
| Easy to use | +++ | ++ | ++ | + | + | – | – |
| Speed of activation | ++ | + | ++ | ++ | ++ | – | – |
HotStarTaq Plus DNA Polymerase specifications
Concentration: 5 units/µlRecombinant enzyme: YesSubstrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTPExtension rate: 2–4 kb/min at 72°CHalf-life: 10 min at 97°C ; 60 min at 94°CAmplification efficiency: ≥105 fold5"–>3" exonuclease activity: YesExtra A addition: Yes3"–>5" exonuclease activity: NoContaminating nucleases: NoContaminating RNases: NoContaminating proteases: NoSelf-priming activity: No
HotStarTaq Plus DNA Polymerase provides the unrivaled performance of HotStarTaq DNA Polymerase with a shortened activation time of just 5 minutes.
HotStarTaq Plus DNA Polymerase, a modified form of QIAGEN Taq DNA Polymerase, is supplied in an inactive state that has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during PCR setup and the initial PCR cycle (see figures "Highest specificity" and "Higher specificity with different primer-template systems"). HotStarTaq Plus DNA Polymerase is activated by a short 5-minute incubation at 95°C which can be easily incorporated into any existing thermal-cycler program.
QIAGEN PCR Buffer
QIAGEN PCR Buffer maintains specific amplification in every cycle of PCR by promoting a high ratio of specific-to-nonspecific primer binding during the annealing step in each PCR cycle (see figure "Increased specificity ofprimer annealing"). Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the buffer provides stringent primer-annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is therefore often minimal or not required.
CoralLoad PCR Buffer
HotStarTaq Plus DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as the conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes — an orange dye and a red dye — that facilitate estimation of DNA migration distance and optimization of agarose gel run time (see figure "CoralLoad PCR Buffer").The buffer ensures improved pipetting visibility and enables direct loading of PCR products onto a gel, for enhanced convenience.
Q-Solution
Q-Solution, an innovative PCR additive that facilitates amplification of difficult templates by modifying the melting behavior of DNA, is also provided with HotStarTaq DNA Polymerase. This unique reagent improves suboptimal PCR caused by templates that have a high degree of secondary structure or GC-rich templates (see figure "Amplification of difficult templates"). Unlike other commonly used PCR additives such as DMSO, Q-Solution is used at just one working concentration, is nontoxic, and PCR purity is guaranteed. Adding Q-Solution to the PCR does not compromise PCR fidelity.
HotStarTaq Plus DNA Polymerase is highly suitable for a wide variety of applications, including challenging applications such as amplification of:
- Complex genomic templates
- Complex cDNA templates (e.g., RT-PCR)
- Very low-copy targets (e.g., single-cell PCR)
- Reactions with multiple primer pairs
Specifications
Features | Specifications |
| Applications | PCR, RT-PCR, Complex genomic templates, very low-copy targets |
| Enzyme activity | 5" -> 3" exonuclease activity |
| Mastermix | No |
| Reaction type | PCR amplification |
| Real-time or endpoint | Endpoint |
| Sample/target type | Genomic DNA and cDNA |
| Single or multiplex | Single |
| With/without hotstart | With hotstart |
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