IDH1/2 RGQ PCR Kit
- Accurate detection of 7 IDH1 and 5 IDH2 mutations using PCR clamping
- Sensitive identification of 3 mutationsusing ARMS PCR technology
- A standardized assay with ready-to-use solutions
- An easy workflow with the Rotor-Gene Q instrument
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Cat No./ID:873001 IDH1/2 RGQ PCR Kit (20) Log in to see pricing For 20 reactions: 9 Primer and Probe Mixes, WT Control, Positive Control, Master Mix, Nuclease-Free Water |
Product Details
The IDH1/2 RGQ PCR Kit provides reagents to perform 9 separate amplification reactions for detection of 12 mutations:
- 3 total amplification reactions of codons 132 and 100 of the IDH1 gene and of codon 172 of the IDH2 gene
- 3 mutation amplification reactions of codons 132 and 100 of the IDH1 gene and of codon 172 of the IDH2 gene
- 3 mutation-specific amplification reactions of IDH1 R132H, IDH1 R132C, and IDH2 R172K mutations
| Gene | Mutation | Base change | Cosmic ID |
|---|---|---|---|
| IDH1 | Arg132His | 395G>A | COSM28746 |
| Arg132Cys | 394C>T | COSM28747 | |
| Arg132Ser | 394C>A | COSM28748 | |
| Arg132Gly | 394 C>G | COSM28749 | |
| Arg132Leu | 395G>T | COSM28750 | |
| Arg132Val | 394_395 CG>GT | COSM28751 | |
| Arg100Gln | 299G>A | COSM88208 | |
| IDH2 | Arg172Lys | 515G>A | COSM33733 |
| Arg172Met | 515G>T | COSM33732 | |
| Arg172Trp | 514A>T | COSM34039 | |
| Arg172Ser | 516G>T | COSM34090 | |
| Arg172Gly | 514A>G | COSM33731 |
Total reaction mixes
The Total Primers and Probe Mixes (PPM-Total) use primers and probes to amplify both mutated and wild-type target sequences.
Mutation detection reaction mixes
The mutation detection primers and probe mixes combine primers and probes, to amplify both mutated and wild-type target sequences, plus an oligonucleotide, 3" blocked with the addition of a phosphate group to prevent elongation (PCR clamping), which is specific to the wild-type target sequence.
When the PCR template contains the wild-type sequence, the 3"-phosphate oligonucleotide will dominate over PCR primer binding due to higher affinity. There is no or low extension by the DNA polymerase and no or low amplification is observed.
When a mutated sequence is present, PCR primer binding will dominate over the 3"-phosphate oligonucleotide binding and amplification will proceed.
Mutation identification reaction mixes
Allele-specific amplification is achieved by ARMS (Amplification Refractory Mutation System), which exploits the ability of the DNA polymerase to distinguish between a match and a mismatch at the 3" end of a PCR primer.
When the PCR primer is fully matched, the amplification proceeds with full efficiency. When the 3" base is mismatched, only low-level background amplification occurs.
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