Overview
Product name
Human MMP8 ELISA KitSee all MMP8 kitsDetection method
ColorimetricPrecision
Intra-assay Sample n Mean SD CV% Overall 8 1.7% Inter-assay Sample n Mean SD CV% Overall 3 12.2% Sample type
Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasmaAssay type
Sandwich (quantitative)Sensitivity
19.5 pg/mlRange
78 pg/ml -5000 pg/mlRecovery
Sample specific recovery Sample type Average % Range Saliva 99.7 86%- 114% Serum 84 81%- 86% Hep Plasma 86 82%- 90% EDTA Plasma 82 79%- 85% Cit plasma 85 83%- 87% Assay time
1h30mAssay duration
One step assaySpecies reactivity
Reacts with:HumanProduct overview
Human MMP8 ELISA Kit (ab219050) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP8 protein in cit plasma, edta plasma, hep plasma, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP8 with 19.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less- High sensitivity, specificity and reproducibility from superior antibodies- Fully validated in biological samples- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Notes
Matrix metalloproteinase-8 (MMP8), also known as neutrophil collagenase and PMNL collagenase, is a zinc and calcium binding protein encoded by the gene MMP-8. MMP8 belongs to the matrix metalloproteinase family which are involved in the degradation of extracellular matrix, and are involved in embryonic development, reproduction, and tissue remolding. MMP8 is stored inactive in granules within neutrophils, and upon secretion is activated by cleavage of a peptide through the dissociation of a conserved cysteine from a zinc ion. MMP8 degrades type I, II, and III collagens, and the protein contains four hemopexin regions.
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
Storage instructions
Store at +4°C. Please refer to protocols.Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml 10X Human MMP8 Capture Antibody 1 x 600µl 10X Human MMP8 Detector Antibody 1 x 600µl Human MMP8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMBDevelopment Solution 1 x 12ml Research areas
- Cardiovascular
- Angiogenesis
- Adhesion / ECM
- Matrix Metalloproteinases
- MMP
- Signal Transduction
- Cytoskeleton / ECM
- Extracellular Matrix
- ECM Enzymes
- MMP
- Cancer
- Invasion/microenvironment
- ECM
- Extracellular matrix
- MMPs
- Cell Biology
- Proteolysis / Ubiquitin
- Proteolytic enzymes
- Metalloprotease
- MMPs
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs
- Cardiovascular
- Atherosclerosis
- Vascular Inflammation
- Leukocyte recruitment
- Other
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Atherosclerotic proteins ELISA kits
- Kits/ Lysates/ Other
- Kits
- ELISA Kits
- ELISA Kits
- Tumor biomarkers ELISA kits
Function
Can degrade fibrillar type I, II, and III collagens.Tissue specificity
Neutrophils.Sequence similarities
Belongs to the peptidase M10A family.Contains 4 hemopexin-like domains.Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.- Information by UniProt
Alternative names
- CLG 1
- CLG1
- Collagenase 1
see allDatabase links
- Entrez Gene:4317 Human
- Omim:120355 Human
- SwissProt:P22894 Human
- Unigene:161839 Human
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