The NucleoSpin 8 Plasmid and NucleoSpin 8 Plasmid Core Kits are designed to purify up to 20 µg of high-copy plasmids from 1–5 ml of E. coli overnight cultures (preferably grown in LB medium). The NucleoSpin Plasmid Binding Strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.
Bacteria are harvested in an initial centrifugation step. After resuspension of the pelleted bacteria and alkaline cell lysis, the crude bacterial lysates are cleared by centrifugation or loaded onto NucleoSpin Plasmid Filter Strips arranged on the NucleoVac 96 vacuum manifold.
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