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Immunoway/Actinin-α1 Polyclonal Antibody/50ug 100ug/YT0100

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Immunoway/Actinin-α1 Polyclonal Antibody/50ug 100ug/YT0100

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Immunoway

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YT0100

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4000-520-616

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Actinin-α1 Polyclonal Antibody

Catalog No.：YT0100
Applications：WB,ELISA
Reactivity：Human,Mouse,Rat

Data Sheet
MSDS
Support

Description
References ( 0 )
Protocol

Gene Name：
ACTN1

Protein Name：
Alpha-actinin-1

Human Gene Id：
87

Human Swiss Prot No：
P12814

Mouse Gene Id：
109711

Mouse Swiss Prot No：
Q7TPR4

Rat Gene Id：
81634

Rat Swiss Prot No：
Q9Z1P2

Immunogen：
Synthesized peptide derived from Actinin-α1 . at AA range: 1-80

Specificity：
Actinin-α1 Polyclonal Antibody detects endogenous levels of Actinin-α1 protein.

Formulation：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：
Rabbit

Dilution：
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：
1 mg/ml

Storage Stability：
-20°C/1 year

Other Name：
ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1

MolecularWeight(Da)：
103058

Observed Band(KD)：
103

Background：
actinin alpha 1(ACTN1)Homo sapiensAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Function：
function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP.,

Subcellular Location：
stress fiber,ruffle,extracellular region,extracellular space,intracellular,cytoplasm,cytosol,actin filament,plasma membrane,brush border,cell-cell junction,fascia adherens,focal adhesion,Z disc,platelet

Expression：
Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue,

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