- SynonymOV16,OV-16 antigen
- SourceOnchocerca volvulus OV16, GST Tag (OV6-V51G4) is expressed from E.coli cells. It contains AA Lys 17 - Asp 197 (Accession # P31729-1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a GST tag at the N-terminus.
The protein has a calculated MW of 46.8 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Onchocerca volvulus OV16, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundThe OV16 antigen, localizes to the hypodermis, cuticle, and uterus of female O. volvulus is regarded as a highly sensitive and specific antigen for detection of IgG4 in people with prior exposure to onchocerciasis. It has an open reading frame that encodes a 152- amino acid polypeptide containing a 16-amino acid putative signal peptide. Enzyme-linked Immunosorbent Assay serological testing of OV-16 antigen is a diagnostic tool for determining effective elimination of the parasite.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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