ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with chromatin bound non-specifically which can make data interpretation difficult. To solve these problems, Active Motif offers species-specific ChIP-IT^® Control Kits for real time or endpoint PCR. Control kits are ideal for ChIP antibody and primer set validation when run in parallel with your test samples. The control kit will help to confirm the chromatin preparation and immunoprecipitation procedures worked properly and allow you to assess the quality of your test antibody and primer sets.

The ChIP-IT^® Control qPCR Kits include a positive control antibody, a bridging antibody to enhance binding affinity of mouse monoclonal antibodies, a negative control antibody to evaluate non-specific binding and species-specific positive and negative control qPCR primers. The positive and negative control primer sets enable direct comparison of gene targets for the same antibody immunoprecipitation. This eliminates the variation that is seen when comparing a ChIP antibody result to that of a negative control IgG antibody, as each have different binding affinities.

Our original ChIP-IT^® Control Kits are designed for use in endpoint PCR. Each kit provides positive and negative control antibodies, bridging antibody to enhance the binding affinity of mouse monoclonal antibodies, a positive control PCR primer set as well as a PCR buffer/DNA loading dye that makes your PCR reactions ready to run on the gel straight out of the thermocycler.

ActiveMotif公司是一家致力于细胞生物学和分子生物学研究用试剂的开发和生产的专业公司，是世界上最大的转录因子试剂生产商。其细胞生物学产品涉及：蛋白质转染试剂、氧化一氮定量试剂盒、转录因子ELISA试剂盒、细胞核提取试剂盒、染色试剂、细胞提取物及抗体等；分子生物学产品涉及：mRNA和总RNA的提取、CompetentE.coli(感受态细胞)、E.coli表达系统、蛋白纯化系统、基因快速富集系统、DNA分离纯化系统、CDNA合成、RT-PCR试剂盒以及其他辅助试剂。

随着人类以及其他各种动物、植物、微生物基因组DNA序列的破译，ActiveMotif公司开始致力于细胞功能和调控、基因相互作用及其编码蛋白质的研究，为全球人员提供高质量的产品和优质的服务。近年来，随着癌症发病率的上升，人们越来越多地关注癌症治疗和防治的问题。研究发现，在基因的DNA序列没有发生改变的情况下，通过基因修饰、蛋白质相互作用等改变了遗传基因的功能和特性，并通过细胞分裂和增殖来影响遗传。因此，相关研究主要集中在基因的表达和调控等表观遗传学研究领域。

ActiveMotif公司拥有一系列表观遗传学研究的相关产品，主要包括：染色质免疫共沉淀检测试剂、DNA甲基化检测及收集试剂、组蛋白纯化试剂、检测组蛋白修饰的抗体、组蛋白乙酰转移酶活性检测试剂、核蛋白抽提试剂、有丝分裂指数分析试剂及转录因子ELISA试剂等。

ActiveMotif公司提供创新性的生物技术应用于多个研究领域，其产品对发现、破译和了解复杂的细胞功能提供了有力的帮助，并不断开发新产品，为生物工程领域的研究提供了可靠保证。