The Next Gen DNA Library Kit is designed to prepare Next generation sequencing (NGS) libraries from double-stranded input DNA for use with Illumina®sequencing platforms. Libraries can be generated from as little as 10 pg of high-quality genomic DNA, ChIP DNA, FFPE DNA or cell-free DNA (cfDNA), which is 500-fold more sensitive than other library preparation kits. Additionally, libraries can be prepared from 100 ng DNA if preparing PCR-free libraries. | Schematic of a completed MID tagged and indexed DNA library molecule. |
Next Gen DNA Library Kit is used in combination with the Next Gen Indexing Kit, which contains 16 unique index adapters that may be used individually, or in combination to multiplex samples together during cluster generation for co-sequencing on the same Illumina flow cell. The advantage of the Next Gen DNA Library preparation is the inclusion of molecular identifiers (MIDs) to enable accurate removal of PCR duplicates from single read sequencing. This helps to increase the number of unique alignments for more accurate data sets. For more information please click on the Overview tab below. To download the protocol, please select the Documents tab. |
Active Motif"s Next Gen DNA Library Kit and Next Gen Indexing Kit are powered by Swift Biosciences. |  |
ActiveMotif公司是一家致力于细胞生物学和分子生物学研究用试剂的开发和生产的专业公司，是世界上最大的转录因子试剂生产商。其细胞生物学产品涉及：蛋白质转染试剂、氧化一氮定量试剂盒、转录因子ELISA试剂盒、细胞核提取试剂盒、染色试剂、细胞提取物及抗体等；分子生物学产品涉及：mRNA和总RNA的提取、CompetentE.coli(感受态细胞)、E.coli表达系统、蛋白纯化系统、基因快速富集系统、DNA分离纯化系统、CDNA合成、RT-PCR试剂盒以及其他辅助试剂。
随着人类以及其他各种动物、植物、微生物基因组DNA序列的破译，ActiveMotif公司开始致力于细胞功能和调控、基因相互作用及其编码蛋白质的研究，为全球人员提供高质量的产品和优质的服务。近年来，随着癌症发病率的上升，人们越来越多地关注癌症治疗和防治的问题。研究发现，在基因的DNA序列没有发生改变的情况下，通过基因修饰、蛋白质相互作用等改变了遗传基因的功能和特性，并通过细胞分裂和增殖来影响遗传。因此，相关研究主要集中在基因的表达和调控等表观遗传学研究领域。
ActiveMotif公司拥有一系列表观遗传学研究的相关产品，主要包括：染色质免疫共沉淀检测试剂、DNA甲基化检测及收集试剂、组蛋白纯化试剂、检测组蛋白修饰的抗体、组蛋白乙酰转移酶活性检测试剂、核蛋白抽提试剂、有丝分裂指数分析试剂及转录因子ELISA试剂等。
ActiveMotif公司提供创新性的生物技术应用于多个研究领域，其产品对发现、破译和了解复杂的细胞功能提供了有力的帮助，并不断开发新产品，为生物工程领域的研究提供了可靠保证。
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