Format
96-Well Plate
Species Reactivity
Homo sapiens (Human)
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20 ng/mL
Sensitivity
0.104 ng/mL
Estimated Turnaround Time
7-11 business days
Target Name
Death Inducer Obliterator 1
Target Synonyms
BYE1; DATF1; DIDO2; DIDO3; DIO1; Death Associated Transcription Factor 1
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death Inducer Obliterator 1 (DIDO1) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death Inducer Obliterator 1 (DIDO1) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100. Intra-assay: CV<10%. inter-assay:=""><>
Detection Method
Colorimetric
Assay Time
3 hours
Application
ELISA
Assay Type
Double-antibody Sandwich
Shipping Condition
Ice packs
Storage
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
Q9BTC0
Specificity
This assay has high sensitivity and excellent specificity for detection of Death Inducer Obliterator 1 (DIDO1).
Cross Activity
This assay has high sensitivity and excellent specificity for detection of Death Inducer Obliterator 1 (DIDO1).
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Inducer Obliterator 1 (DIDO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Inducer Obliterator 1 (DIDO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Inducer Obliterator 1 (DIDO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Death Inducer Obliterator 1 (DIDO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90 µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50 µL Stop Solution. Read at 450nm immediately.
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Research Area
Apoptosis;
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