Active Motif offers a wide variety of recombinant histones that include site- and degree-specific modifications such as methylation, acetylation and phosphorylation. The combination of histone post-translational modifications form the basis of the “histone code” that serves to regulate a variety of nuclear functions, including interactions with chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair. Each recombinant histone is prepared using one of two patented technologies: Expressed Protein Ligation (EPL) or Methylated Lysine Analog (MLA). We also offer a subset of our histone H3 proteins that have been biotinylated for use in FRET assays and other capture techniques.

With our MLA technology, methylated histones are generated via a chemical alkylation reaction that introduces a methyl-lysine analog at the desired lysine location, giving us precise control over the site and degree of methylation. Alternatively, the EPL technology can be used to generate methylated, acetylated and phosphorylated histones. Using EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired modifications. This ligation reaction maintains the native histone bonds. Both methods produce proteins that are validated to be over 98% pure. For more information on the EPL and MLA technologies, click on the EPL and MLA Technologies tab below.

Active Motif also offers a number of biotinylated recombinant histone H3 proteins. Biotin is linked either to unmodified or modified recombinant H3 protein at its N-terminus via a carbon linker. The addition of biotin enables the recombinant histones to be used as a substrate for capture of protein binding interactions. Biotinylated histone H3 proteins are also ideal substrates for homogenous FRET assays. Simply incubate the recombinant histone with the enzyme of interest and detect using streptavidin-coated donor beads and antibody-conjugated acceptor beads.

A complete list of recombinant histones is shown below. Click on the protein name to see complete information.

ActiveMotif公司是一家致力于细胞生物学和分子生物学研究用试剂的开发和生产的专业公司，是世界上最大的转录因子试剂生产商。其细胞生物学产品涉及：蛋白质转染试剂、氧化一氮定量试剂盒、转录因子ELISA试剂盒、细胞核提取试剂盒、染色试剂、细胞提取物及抗体等；分子生物学产品涉及：mRNA和总RNA的提取、CompetentE.coli(感受态细胞)、E.coli表达系统、蛋白纯化系统、基因快速富集系统、DNA分离纯化系统、CDNA合成、RT-PCR试剂盒以及其他辅助试剂。

随着人类以及其他各种动物、植物、微生物基因组DNA序列的破译，ActiveMotif公司开始致力于细胞功能和调控、基因相互作用及其编码蛋白质的研究，为全球人员提供高质量的产品和优质的服务。近年来，随着癌症发病率的上升，人们越来越多地关注癌症治疗和防治的问题。研究发现，在基因的DNA序列没有发生改变的情况下，通过基因修饰、蛋白质相互作用等改变了遗传基因的功能和特性，并通过细胞分裂和增殖来影响遗传。因此，相关研究主要集中在基因的表达和调控等表观遗传学研究领域。

ActiveMotif公司拥有一系列表观遗传学研究的相关产品，主要包括：染色质免疫共沉淀检测试剂、DNA甲基化检测及收集试剂、组蛋白纯化试剂、检测组蛋白修饰的抗体、组蛋白乙酰转移酶活性检测试剂、核蛋白抽提试剂、有丝分裂指数分析试剂及转录因子ELISA试剂等。

ActiveMotif公司提供创新性的生物技术应用于多个研究领域，其产品对发现、破译和了解复杂的细胞功能提供了有力的帮助，并不断开发新产品，为生物工程领域的研究提供了可靠保证。