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Sino Biological/CXCL5 ELISA Kit, Human/1/KIT10889

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Sino Biological/CXCL5 ELISA Kit, Human/1/KIT10889

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Sino Biological

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KIT10889

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Description
Related Products
Citations

CXCL5 ELISA Kit, Human: General Information

Product name

CXCL5 ELISA Kit, Human

Assay type

Solid Phase Sandwich ELISA (quantitative)

Conjugate

HRP

Sample type

Serum,Cell supernatant,Plasma

Sensitivity

1.44 pg/mL

Assay range

4.69-300 pg/mL

Specificity

Recognizes both recombinant and natural Human CXCL5

Interference

Preparations ofthe factors listed below at 50 ng/mL in a mid-range recombinant human CXCL5 control were assayed for interference. No significant interference was observed.

Precision

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
N	20	20	20	20	20	20
Mean(pg/mL)	61.94	99.46	191.27	59.84	102.41	199.19
SD	2.65	5.03	7.23	1.48	3.62	4.33
CV(%)	4.3%	5.1%	3.8%	2.5%	3.5%	2.2%

	Intra-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	61.94	99.46	191.27
SD	2.65	5.03	7.23
CV(%)	4.3%	5.1%	3.8%
	Inter-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	59.84	102.41	199.19
SD	1.48	3.62	4.33
CV(%)	2.5%	3.5%	2.2%

Recovery

The recovery of Human CXCL5 spiked to different levels throughout the range of the assay in related matrices was evaluated.

Sample	Average % Recovery	Range
serum (n=3)	110	105-119%
supernatant (n=3)	121	112-129%
plasma (n=3)	106	103-112%

Sample
serum (n=3)
Average % Recovery
110
Range
105-119%
Sample
supernatant (n=3)
Average % Recovery
121
Range
112-129%
Sample
plasma (n=3)
Average % Recovery
106
Range
103-112%

Linearity

Serum

	Recovery of detected
1:2	110%
1:4	103%
1:8	97%
1:16	72%

Supernatant

	Recovery of detected
1:2	99%
1:4	97%
1:8	97%
1:16	90%

Plasma

	Recovery of detected
1:2	104%
1:4	105%
1:8	95%
1:16	84%

Materials provided

1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution

Product overview

This CXCL5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL5 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human CXCL5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Shipping

This ELISA Kit is shipped at ambient temperature.

Storage

Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA

CXCL5 ELISA Kit, Human: Images

Human CXCL5 ELISA Kit Standard Curve 22523

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

This assay recognizes both recombinant and natural Human CXCL5. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CXCL5 ELISA Kit, Human: Synonyms

ENA-78 ELISA Kit, Human; SCYB5 ELISA Kit, Human

CXCL5 Background Information

CXCL5 is a small cytokine belonging to the CXC chemokine family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. CXCL5 is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. It also can be detected in eosinophils, and can be inhibited with the type II interferon. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis. It stimulates the chemotaxis of neutrophils possesses angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.

Full Name

chemokine (C-X-C motif) ligand 5

Related Pathways

IL17 signaling pathway

References

Dawes JM, et al. (2011) CXCL5 Mediates UVB Irradiation-Induced Pain. Sci Transl Med. 3(90): 90ra60.
O"Donovan N, et al. (1999) Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84(1-2):39-42.
Persson T, et al. (2003) Expression of the neutrophil-activating CXC chemokine ENA-78/CXCL5 by human eosinophils. Clin Exp Allergy. 33(4):531-7.

CXCL5 Matched ELISA Antibody Pair Set,Human
Assay Range(pg/ml):3.91-250
SEK10889

Schwann cells promote lung cancer proliferation by promoting the M2 polarization of macrophages
Author
Zhou, Y;Li, J;Han, B;Zhong, R;Zhong, H;
Year
2020
Journal
Cellular Immunology
Application
ELISA
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