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Sino Biological/PCSK9 Matched ELISA Antibody Pair Set,Human/1/SEK10594
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PCSK9 Matched ELISA Antibody Pair Set,Human General Information
Product name
PCSK9 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human PCSK9
Assay range
468.75-30000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human PCSK9 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10594-MM15)2. Detection Antibody 0.2 mg/mL of mouse anti-Human PCSK9 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.2 μg/mL in Dilution Buffer before use. (Catalog: # 10594-MM20)3. Standard Each vial contains 960 ng of recombinant Human PCSK9. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 30000 pg/mL is recommended.
Product overview
This PCSK9 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human PCSK9 . It contains Human PCSK9 capture antibody, Human PCSK9 detector antibody (HRP)and a highly purifiedrecombinant Human PCSK9 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
PCSK9 Matched ELISA Antibody Pair Set,Human Images
PCSK9 Matched ELISA Antibody Pair Set,Human: Synonyms
FH3 Matched ELISA Antibody Pair Set, Human; HCHOLA3 Matched ELISA Antibody Pair Set, Human; LDLCQ1 Matched ELISA Antibody Pair Set, Human; NARC-1 Matched ELISA Antibody Pair Set, Human; NARC1 Matched ELISA Antibody Pair Set, Human; PC9 Matched ELISA Antibody Pair Set, Human
PCSK9 Background Information
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Full Name
proprotein convertase subtilisin/kexin type 9
Research Areas
- Serine Proteases and Regulators
References
- Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
- Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
- Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
- Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
- Ascorbic acid enhances low-density lipoprotein receptor expression by suppressing proprotein convertase subtilisin/kexin 9 expressionAuthorWang, D;Yang, X;Chen, Y;Gong, K;Yu, M;Gao, Y;Wu, X;Hu, H;Liao, C;Han, J;Duan, Y;Year2020JournalJ. Biol. Chem.PubMed ID: 32913121FoldedExpand
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