Addgene/lentiCRISPR - EGFP sgRNA 4/1unit/51763_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

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Addgene/lentiCRISPR - EGFP sgRNA 4/1unit/51763

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Addgene/lentiCRISPR - EGFP sgRNA 4/1unit/51763

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Addgene

货号 /

51763

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4000-520-616

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Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	51763	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
lentiCRISPR (pXPR_001), Addgene plasmid #49535
(Search Vector Database)
Backbone manufacturer
Zhang lab
Vector type
Mammalian Expression, Lentiviral, CRISPR
Selectable markers
Puromycin

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
Stbl3
Growth instructions
Use SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
Copy number
High Copy

Gene/Insert1

Gene/Insert name
Cas9
Alt name
S. pyogenes CRISPR-Cas9
Species
Synthetic
Insert Size (bp)
4200
PromoterEFS

Cloning Informationfor Gene/Insert 1

Cloning methodRestriction Enzyme
5′ cloning siteBamHI(not destroyed)
3′ cloning siteNheI(not destroyed)
5′ sequencing primerGGTACAGTGCAGGGGAAAGA
3′ sequencing primerTGCCCTCCAAATATGTGAACT

Gene/Insert2

Gene/Insert name
Puromycin resistance
Alt name
puromycin N-acetyl-transferase
Alt name
PAC
Insert Size (bp)
600
PromoterEFS

Cloning Informationfor Gene/Insert 2

Cloning methodRestriction Enzyme
5′ cloning siteNheI(not destroyed)
3′ cloning siteMluI(not destroyed)
5′ sequencing primerTGCTGCTACTAAGAAAGCTGGTC

Gene/Insert3

Gene/Insert name
EGFP sgRNA 4
Alt name
EGFP targeting RNA element #4 (with +85 chimeric RNA)
Species
Synthetic
Insert Size (bp)
96
PromoterU6

Cloning Informationfor Gene/Insert 3

Cloning methodRestriction Enzyme
5′ cloning siteBsmBI(destroyed during cloning)
3′ cloning siteBsmBI(destroyed during cloning)
5′ sequencing primer#140 hU6-F

Resource Information

Terms and Licenses
- UBMTA
Industry Terms
- Not Available to Industry
Articles Citing this Plasmid
- 2 References

Depositor Comments

gRNA target sequence GGAGCGCACCATCTTCTTCA

These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids.

Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check images/Addgene/ for the most up-to-date information.

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