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Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 36158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepLVX-Puro(Search Vector Database)
- Backbone manufacturerClontech
- Vector typeMammalian Expression, Lentiviral
- Selectable markersPuromycin
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameSec23A
- SpeciesH. sapiens (human)
- Entrez GeneSEC23A(a.k.a. CLSD, hSec23A)
- PromoterCMV
- Tag/ Fusion Protein
- mRuby (N terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteSal1(destroyed during cloning)
- 3′ cloning siteXba1(not destroyed)
- 5′ sequencing primerCMV
- 3′ sequencing primerunknown (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- Seqbuilder file of the sequence
- A portion of this plasmid was derived from a plasmid made bymRuby was synthesized to our requirements by Eurofins MWG
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 2 References
Depositor Comments
The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usageadaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript IISK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplifiedby PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)and cloned into pLVX-Puro–mRuby.
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