
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 105866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepGEM T-easy(Search Vector Database)
- Backbone manufacturerPromega
- Backbone sizew/o insert(bp)3017
- Total vector size (bp)3620
- Vector typePlant Expression, CRISPR, Synthetic Biology ; Subcloning
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameU6-26p::sgRNA scaffold
- gRNA/shRNA sequencecan be inserted via BbsI sites
- SpeciesA. thaliana (mustard weed), Synthetic
Resource Information
- Supplemental Documents
- pFH6 new genebank.gb
- Cloning Protocol for pFH6_new and pUB-Cas9
- A portion of this plasmid was derived from a plasmid made byPeter Hegemann (HU Berlin)
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%.
For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( images/Addgene/e2384 ).
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