Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Milk, Saliva, Stool

Sample Requirements

Serum, Plasma (EDTA), BALF, Breast milk, Saliva: 5 µl/well

CSF, Urine, Stool extract: 20 µl/well

• For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml)
• Special Dilution Buffer is needed for measurement of human lactoferrin in stool extract and is not included.
  For protocol for preparation of stool extracts and other details, please contact us at info@biovendor.com

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box)

Calibration Curve

Calibration Range

2.5–80 ng/ml

Limit of Detection

1.1 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 3.3 %

Inter-assay (Run-to-Run)

n = 6; CV = 5.4 %

Spiking Recovery

Serum: 96.6 %
Plasma (EDTA): 103.2 %
BALF: 104.7 %
CSF: 102.3 %
Urine: 104.4 %
Stool extract: 89.7 %

Dilutation Linearity

Serum: 94.6 %
Plasma (EDTA): 97.5 %
BALF: 88.3 %
CSF: 99.5 %
Urine: 98.4 %
Breast milk: 97.1 %
Saliva: 97.1 %
Stool extract: 94.9 %