The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.
The Cellartis Hepatocyte Differentiation Kit generates highly homogeneous hepatocyte cultures starting from definitive endoderm (DE) cells. The resulting hepatocytes show the expected expression of relevant metabolic enzymes, making them suitable for drug metabolism studies and toxicity screening. The Cellartis Hepatocyte Differentiation Kit contains sufficient maintenance medium to perform experiments in an 11-day time window.
There are several options for source materials to generate functional hepatocytes: your own DE cells, our DE cells, or DE cells you generate using the Cellartis Definitive Endoderm Differentiation Kit. This kit has been successfully tested with DE cells derived from 28 different human pluripotent cell lines. Our cells, Cellartis Definitive Endoderm Cells (from ChiPSC18), are suitable as the starting material or as a positive control. If you desire a customized (standard, patient-specific, or disease-specific) genetic background for your DE cells, use the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. # Y30055).
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
GM实验：试剂说明书上要求20分钟显色，10分钟的时候看一下，差不多就可以加终止液，但是我的标曲显色特别快，2分钟最低浓度蓝色就就深了，此时样本颜色还很浅很浅，我只能一块儿加终止液。所以，标曲显色过快，可能是什么原因？
（ps:第一列是标曲，后面是样本）
