ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- Product No.CEA551Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.54pg/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 529 For more details, please contact local distributors!US$ 756 For more details, please contact local distributors!US$ 3402 For more details, please contact local distributors!US$ 6426 For more details, please contact local distributors!US$ 52920 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.
Recovery of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 2, Basic (FGF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 2, Basic (FGF2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-101 | 95 |
| EDTA plasma(n=5) | 86-95 | 91 |
| heparin plasma(n=5) | 79-99 | 81 |
Precision of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 2, Basic (FGF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-99% | 91-101% | 97-104% | 87-94% |
| EDTA plasma(n=5) | 86-101% | 91-102% | 78-94% | 81-96% |
| heparin plasma(n=5) | 95-104% | 79-102% | 84-98% | 91-98% |
Stability of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle of the ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 2, Basic (FGF2) and unlabeled Fibroblast Growth Factor 2, Basic (FGF2) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the sample.
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Related products
| Catalog No. | Organism species: Bos taurus; Bovine (Cattle) | Applications (RESEARCH USE ONLY!) |
| RPA551Bo01 | Recombinant Fibroblast Growth Factor 2, Basic (FGF2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA551Bo01 | Active Fibroblast Growth Factor 2, Basic (FGF2) | Cell culture; Activity Assays. |
| PAA551Bo01 | Polyclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2) | WB; IHC; ICC; IP. |
| MAA551Bo21 | Monoclonal Antibody to Fibroblast Growth Factor 2, Basic (FGF2) | WB; IHC; ICC; IP. |
| CEA551Bo | ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
