Affymetrix recommends the Titanium DNA Amplification Kit for use with its CytoScan HD, CytoScan 750K* Arrays, the Genome-Wide Human SNP Array 6.0, and the original GeneChip Human Mapping 250K and 500K Array Sets. Affymetrix recommends Titanium Taq DNA Polymerase for genome amplification on the basis of its superior performance and quality.
*CytoScan 750K Arrays are not available from Affymetrix in the USor Canada.
Affymetrix recommends the Titanium DNA Amplification Kit for use with its CytoScan HD, CytoScan 750K* Arrays, the Genome-Wide Human SNP Array 6.0, and the original GeneChip Human Mapping 250K and 500K Array Sets. Affymetrix recommends Titanium Taq DNA Polymerase for genome amplification on the basis of its superior performance and quality.
The Titanium DNA Amplification Kit features Titanium Taq DNA Polymerase, a 5"-exonuclease deficient Taq polymerase blended with TaqStart Antibody for automatic hot-start PCR. The kits also contain an optimized buffer mix (containing MgCl2), purified dNTP mix, and our proprietary RNase-free GC-Melt Reagent, which improves PCR amplification and yield when using templates with high GC-content or complex secondary structure.
The Titanium DNA Amplification Kit was designed in collaboration with Affymetrix scientists and comes in two sizes. Cat. # 639240 contains enough reagents for 300 reactions. Cat. # 639243, recommended for use with CytoScan HD, contains sufficient reagent for 400 reactions. The 300-reaction kit can be used by itself to support the original two-chip format, and screen 96 genomic DNA samples with the Mapping 500K Array Set. The 400-reaction kit can support the single-chip format, and screen 48 genomic DNA samples with the SNP Array 6.0.
The Titanium DNA Amplification Kit has been functionally validated by Affymetrix scientists for use with their CytoScan HD, CytoScan 750K, SNP Array 6.0, the original Mapping 250K, and 500K Arrays. This extra validation step ensures that the Titanium amplification step will never be a roadblock to your genotyping and cytogenetics success.
*CytoScan 750K Arrays are not available from Affymetrix in the USor Canada.
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采用抗-HBe抗体包被反应板，加入校准品及被测样本，同时加入定量HBeAg中和抗原，经过振荡孵育,洗板后再加入铕标记的抗-HBe，若标本中抗-HBe浓度高，HBeAg将被大量中和，使最后形成的抗-HBe-HBeAg-铕标记抗-HBe复合物减少。增强液（β－NTA）将标记在抗体上的Eu3+解离到溶液中，Eu3+和增强液中的有效成分形成高荧光强度的螯合物，荧光强度和样本中的抗-HBe浓度成反比。
GM实验：试剂说明书上要求20分钟显色，10分钟的时候看一下，差不多就可以加终止液，但是我的标曲显色特别快，2分钟最低浓度蓝色就就深了，此时样本颜色还很浅很浅，我只能一块儿加终止液。所以，标曲显色过快，可能是什么原因？
（ps:第一列是标曲，后面是样本）
