Description:
TheTECO®MucusCollectionSetisacollectionsetforfishmucus,includingvalidatedswabs,sampletubesinasampletuberack,aspecificallydesignedextractionbufferandakitinstruction.TheMucusCollectionSetisavalidatedtoolforrepeatablenon-invasive/non-destructivesamplingofepidermalmucosaforvitellogendetermination.
TheTECO®MucusCollectionSetforfishmucusisavalidatedtoolforrepeatablenon-destructivesamplingofepidermalmucosaforvitellogenindeterminationusingthe:
- TECO®Perch(Perciformes)VitellogeninELISAKit,TE1035
- TECO®CyprinidVitellogeninELISAKit,TE1037
Componentsarereadytouse.
KitComposition:
1: BreakableSwabs,42. Readytouse.
2: ReactionVials,42. Readytouse.
3: ExtractionBuffer,1x25ml Readytouse.
4: RackforReactionVials,1x
I:Kitinstruction,1x
Background:
Vitellogenin(VTG)isoneofthecoreendpointsinscreeningandtestingforendocrinedisruptingchemicalsstandardizedintheOECDGuidelinesforthetestingofchemicalsforestrogenicactivity.Originallybelievedtobeproducedonlyintheliver,severalcelltypeshaverecentlybeenshowntoproduceVTGafterestrogenstimulation,includingthoseoftheepidermalmucosaoffish.
GiventhedestructivenatureoftrADItionalsampling,collectingsuccessivesamplesfromfishhasbeenawidespreadproblem,asforinstanceintheanalysisofvitellogenininductionwhilescreeningandtestingchemicalsubstancesforendocrinedisruptionpotential.Bloodisdifficulttocollect,inparticularwhereverysmallfishareconcerned,orinapproacheswheretheanimalsmustsurvivesampling.Thisisparticularlyimportantinfieldmonitoringinordertoavoidimpactonthepopulationunderinvestigation.
EventhoughtheVTGconcentrationintheskinmucusisanorderofmagnitudelowerthaninbloodserumorinbodyhomogenates(containinglivertissue),theskinmucosaisverywellsuitedasamatrixtodetermineexogenousVTGinductioncausedbyenvironmentalchemicalswithaffinitytotheestrogenreceptors.Mucussamplingwiththeaidofswabsconstitutesanalternativetotraditionalprocedures,avoidinganimalinjurybyusingahighlysensitiveELISAincombinationwithahighqualityextractionbufferandswabsampling,thedeterminationofmucosa-derivedVTGhasthefollowingadvantages:
- Non-destructive,non-invasivesampling
- Definedmatrixwithoutlymphaticfluidcontamination
- Allowstheuseofsmallerfishfortestingandmonitoringpurposes
- Repeatedsamplingallowsforindividualrecordingoftheinductionkinetic
Thesamplingtechniqueleadstohighlycomparableresultswhentestingsamplesfromtheleftandtherightsidefromthesameindividualininter-laboratorytrials.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
2、规范包被操作,吸附是否均匀
3、重复性、可靠性
6、是否提供技术
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
ELISA检测试剂盒是用于体外定性检测人血清或血浆中的抗人类戊型肝炎(HEV)病毒 IgM抗体.