Description:TheNAD-dependentAldehydeDehydrogenase(ALDH)playsavitalroleincellulardetoxification.Itoxidizesvariousaldehydesandgeneratesthecorrespondingcarboxyolicacid.ALDHhavebeenfoundineverycellularcompartment.Basedonitsstructureandfunction,ALDHcomprises3majorclassesinmammals:Class1andClass3(thetumorform)arelocatedinthecytosolandincludebothconstitutiveandinducedforms;Class2islocatedinthemitochondriaandonlyexistsastheconstitutiveform.Inhumans,theALDHsuperfamilyconsistsof19genes.ThemutationofALDHgenes(lossoffunction)causeshumandiseasessuchasTypeIIhyperprolinemia,pyridoxine-dependentseizureandhyperammonemia.RecentstudiesshowthatincreasedALDHactivityleadstoseveraltypesofmalignancies,servesasacancerstemcellMarkerandcorrelateswithpoorprognosis.ThereforetheearlydetectionofALDHactivitylevelscanbeprognosticandguidethetherapeuticstrategies.GenWay"sPicoProbe™AldehydeDehydrogenaseActivityAssayKitisarobusttooltoquantifyALDHenzymaticactivity.Inthisassay,acetaldehydeisoxidizedbyALDHtoformtheNADHwhichcoupleswiththePicoProbetogenerateapotentfluorescence(Ex/Em=535/587).TheALDHfluorometricassaykitis10timesmoresensitivethantheALDHcolorimetricassayandcandetect< 0.05 mU ALDH activity (based on our unit definition) in a variety of samples. Summary: -Detectionmethod-Fluorescence(Ex/Em535/587nm) -Sampletype-CellandTissueculturesupernatants,celllysates,plasmaandserum,aswellasmanyotherBIOLOGicalfluidsetc. -Speciesreactivity-Mammalian -Application-TheALDHfluorometricassaykitis10timesmoresensitivethantheALDHcolorimetricassayandcandetect< 0.05 mU ALDH activity (based on our unit definition) in a variety of samples. Kitcomponents: -ALDHAssayBuffer -Acetaldehyde -PicoProbeTM(inDMSO) -ALDHSubstrateMix(Lyophilized) -ALDHPositiveControl(Lyophilized) -NADHStandard(0.5µmol,Lyophilized) FeaturesandBenefits: -Simpleprocedure -Fastandconvenient -SensitiveassayformeasuringALDHactivityinvariousbiologicalsamplesandthekitisalsosuitableforhighthroughputanalyses. ShippingConditions:Gelpack
Sensitize a 96-well microtiter plate with purified antigen. Prepare a solution of the purified antigen of interest in phosphate buffer (see recipe below) such 查看更多>
IntroductionQuality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of 查看更多>
