Description:

Description: The Fluorogenic SIRT2 Assay Kit is a complete assay system designed to measure Sirtuin 2 (SIRT2) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 32 fluorescent SIRT2 activity measurements. In addition, the kit includes purified SIRT2 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The kit includes sufficient HDAC substrate 1, buffers, NAD+, nicotinamide, and detection reagents for a full 96-well plate. Researchers can test their own SIRT2-containing samples in the remaining wells, or additional SIRT2 enzyme may be ordered separately . The Fluorogenic SIRT2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT2 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing purified SIRT2 enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Components: SIRT2 human recombinant enzyme, 135 ?g; Fluorogenic SIRT substrate 1, 50 ul @ 5 mM; Nicotinamide Adenine Dinucleotide (NAD+), 50 ul @ 50 mM; Nicotinamide, 500 ul @ 10 mM; 2x SIRT Developer (contains 2 mM Nicotinamide), 6 ml; SIRT assay buffer, 10 ul; 1 black, low binding NUNC black microtiter plate. APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. REFERENCES: 1. A. Ito et al. (2001) EMBO J. 20 1331. 2. N.A. Barlev et al. (2001) Mol. Cell 8 1243. 3. A. Ito et al. (2002) EMBO J. 21 6236.

Format: assay kit

Scientific Category: Deacetylase Assay

Additional Information:

Name	Sirt2 Assay Kit
Related Product Names	sirt2, sirtuin 2
Format	Assay Kit
Storage	Mixed storage
Intended Use	Research Use Only