Description:
Description:TheLSD1ChemiluminescentAssayKitisdesignedtodirectlymeasureactivityofhumanlysine-specificdemethylase(LSD1)forscreeningandprofilingapplications.LSD1isachromatin-modifyingenzymethatspecificallyremovesmethylgroupsfrommono-anddimethylatedLysofhistoneH3.LSD1isacriticalcomponentoftranscriptionalregulationviaepigenetichistonemodificationsandisthereforeapotentialtargetfordrugdevelopment.TheLSD1ChemiluminescentAssayKitcomesinaconvenientformat,witha96-wellplateprecoatedwiththemethylatedhistoneH3peptidesubstrate,primaryantibody,thesecondaryHRP-labeledantibody,demethylaseassaybuffer,andpurifiedLSD1for100enzymereactions.Components:LSD1,10?g;Primaryantibody,25?l;SecondaryHRP-labeledantibody110ul;4xLSD1assaybuffer2,3ml;Blockingbuffer50ul;HRPchemiluminescentsubstrate(2components)6mleach;1Blackmicroplateprecoatedwithhistonesubstrate.APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STABILITY:1yearwhenstoredasrecommended.REFERENCES:1.Forneris,F.,Binda,C.,Dall"Aglio,A.,Fraaije,M.W.,Battaglioli,E.,andMattevi,A.J.Biol.Chem.2006;281(46):35289-95.2.Zhou,M.,Diwu,Z.,Panchuk-Voloshina,N.,andHaugland,R.P.Anal.Biochem.1997;253(2):162-8.Format:assaykit
ScientificCategory:Demethylase/Assay
AdditionalInformation:
| Name | LSD1ChemiluminescentAssayKit |
|---|---|
| RelatedProductNames | LSD1ChemiluminescentAssayKit |
| Format | AssayKit |
| Storage | Mixedstorage |
| IntendedUse | ResearchUseOnly |

